Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74667
Título: Molecular diversity of Extended-spectrum β-lactamase-producing Escherichia coli from vultures in Canary Islands
Autores/as: Carvalho, Isabel
Tejedor Junco, María Teresa 
González Martín, Margarita Rosa 
Corbera Sánchez, Juan Alberto 
Suárez Pérez, Alejandro 
Silva, Vanessa
Igrejas, Gilberto
Torres, Carmen
Poeta, Patrícia
Clasificación UNESCO: 241401 Antibióticos
3105 Peces y fauna silvestre
Palabras clave: Antimicrobials
Escherichia coli
Vultures
Canary Islands
Fecha de publicación: 2020
Publicación seriada: Environmental Microbiology Reports 
Resumen: Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug-resistant organisms. The aim of this study was to determine the carriage rate of ESBL-producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free-ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml−1). Colonies with typical morphology of E. coli were identified by MALDI-TOF-MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double-disk tests. The presence of blaCTX-M, blaSHV, blaTEM, blaKPC and blaOXA-48 genes, as well as mcr-1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL-producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried blaCTX-M genes: blaCTX-M-15 (n = 3) and blaCTX-M-55 (n = 2). ESBL-positive isolates were ascribed to phylogenetic group D (two isolates), B1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic-group/ESBL): ST515/B1/CTX-M-15, ST1290/A/CTX-M-15, ST38/D/CTX-M-15, ST457/D/CTX-M-55 and ST6448/B1/CTX-M-55; this suggests a genetic diversity among these isolates. Three CTX-M-15-producing isolates contained the blaTEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL-producing E. coli in vulture chicks from the Canary Islands.
URI: http://hdl.handle.net/10553/74667
ISSN: 1758-2229
DOI: 10.1111/1758-2229.12873
Fuente: Environmental Microbiology Reports [EISSN 1758-2229], v. 12 (5), p. 540-547, (Enero 2020)
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