Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/7464
Title: Isolation of high-lipid content strains of the marine microalga Tetraselmis suecica for biodiesel production by flow cytometry and single-cell sorting
Authors: Montero del Pino, María F. 
Aristizábal, Manuela
García Reina, Guillermo
UNESCO Clasification: 339001 Biotecnología de microalgas
241707 Algología (ficología)
Keywords: Citometry
Fluorescence activated cell sorting (FACS)
Nile Red (NR)
Neutral lipids
Tetraselmis suecica
Issue Date: 2010
Journal: Journal of Applied Phycology 
Abstract: Biodiesel from algae is considered an alternative for a third generation of biofuels. However, most microalgae are not lipogenic during fast growth periods, but high-lipid content occurs at resting stages. Microalgae biomass production for biodiesel needs continuous high volumetric and aerial yields and large amount of neutral lipid in the biomass. These requirements are similar to demanding a marathon runner to be obese. We show that by using cell sorting capabilities of flow cytometers, in combination with the lipid-soluble fluorescent dye Nile Red, we can isolate and select cells with a high and stable lipid content. In our study, we were able to select the equivalent of a stable “fat marathon runner” through three sorting events obtained from wild populations of Tetraselmis suecica.
URI: http://hdl.handle.net/10553/7464
ISSN: 0921-8971
DOI: 10.1007/s10811-010-9623-6
Source: Journal of Applied Phycology [ISSN 0921-8971], v. 23 (6), p. 1053-1057
Rights: by-nc-nd
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