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Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74271
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dc.contributor.authorFoddai, A.en_US
dc.contributor.authorIdini, G.en_US
dc.contributor.authorFusco, M.en_US
dc.contributor.authorRosa, N.en_US
dc.contributor.authorde la Fe, C.en_US
dc.contributor.authorZinellu, S.en_US
dc.contributor.authorCorona, L.en_US
dc.contributor.authorTola, S.en_US
dc.date.accessioned2020-09-06T12:35:29Z-
dc.date.available2020-09-06T12:35:29Z-
dc.date.issued2005en_US
dc.identifier.issn0890-8508en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/74271-
dc.description.abstractThe membrane-protein 81 gene (mb-mp81) of Mycoplasma bovis was cloned, sequenced and compared to membrane-protein 81 gene (ma-mp81) of Mycoplasma agalactine. After alignment of both sequences, specific primers pairs were designed from variable or unchanging nucleotide segments. In this study, we describe the development and optimization of a multiplex-PCR (MPCR) for the rapid detection of M. agalactiae and M. bovis strains. In addition, a simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the restriction enzymes AluI. DraI, RsaI and XbaI, is described to distinguish between both species. The results suggest that MPCR and PCR-RFLP assays could be used as an alternative method in routine diagnosis for rapid and specific simultaneous detection of M. agalactiae and M. bovis.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Probesen_US
dc.sourceMolecular and Cellular Probes [ISSN 0890-8508], v. 19 (3), p. 207-212, (Junio 2005)en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherMycoplasma Agalactiaeen_US
dc.subject.otherMycoplasma Bovisen_US
dc.subject.otherMultiplex-PCRen_US
dc.subject.otherPCR-RFLPen_US
dc.subject.otherMa(Mb)-Mp81 Geneen_US
dc.titleRapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLPen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.mcp.2004.11.007en_US
dc.identifier.scopus15944390235-
dc.identifier.isi000228515500009-
dc.contributor.authorscopusid26647413900-
dc.contributor.authorscopusid8639313200-
dc.contributor.authorscopusid8639313300-
dc.contributor.authorscopusid8639313400-
dc.contributor.authorscopusid9036230000-
dc.contributor.authorscopusid8639313600-
dc.contributor.authorscopusid8639313700-
dc.contributor.authorscopusid55894381700-
dc.description.lastpage212en_US
dc.identifier.issue3-
dc.description.firstpage207en_US
dc.relation.volume19en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid3874369-
dc.contributor.daisngid3316317-
dc.contributor.daisngid5425714-
dc.contributor.daisngid2092292-
dc.contributor.daisngid513821-
dc.contributor.daisngid16405947-
dc.contributor.daisngid34849907-
dc.contributor.daisngid30104518-
dc.description.numberofpages6en_US
dc.utils.revisionSíen_US
dc.contributor.wosstandardWOS:Foddai, A-
dc.contributor.wosstandardWOS:Idini, G-
dc.contributor.wosstandardWOS:Fusco, M-
dc.contributor.wosstandardWOS:Rosa, N-
dc.contributor.wosstandardWOS:de la Fe, C-
dc.contributor.wosstandardWOS:Zinellu, S-
dc.contributor.wosstandardWOS:Corona, L-
dc.contributor.wosstandardWOS:Tola, S-
dc.date.coverdateEnero 2005en_US
dc.identifier.ulpgcSíes
dc.description.jcr1,634
dc.description.jcrqQ3
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
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