Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/73767
Title: Immunohistochemical characterization of normal canine Merkel cells
Authors: Ramirez, Gustavo A.
Herráez Thomas, Pedro Manuel 
Andrada Borzollino, Marisa Ana 
Fernández Rodríguez, Antonio Jesús 
Castro Alonso, Pedro Luis 
Espinosa De Los Monteros Y Zayas, Antonio 
UNESCO Clasification: 310907 Patología
310903 Inmunología
Keywords: Neurofilament Proteins
Skin
Localization
Immunoreactivity
Mammals, et al
Issue Date: 2003
Journal: Merkel Cell: Structure-Development-Function-Cancerogenesis
Conference: 4th International Merkel Cell Symposium
Abstract: Cutaneous Merkel cells (MCs) have been well documented in humans, but less in other mammals. In dogs, there are only a few references about immunohistochemical characterization of MCs. We present the immunohistochemical profile of MCs in the dog for the most reliable antibodies used in human medicine. Tissue samples from several locations were obtained from five adult dogs of both sexes and different age and breed, fixed in 10% buffered formalin, embedded in paraffin wax and cut in 3-mum tissue sections. The ABC method was used with different poly- and monoclonal antibodies. Positive immunoreaction was found in MCs of hair follicles, skin and mucosae of several locations in each dog studied for anticytokeratins 8, 18 and 20, anti-neurofilaments, anti-chromogranin A, anti-neuron-specific enolase, and anti-synaptophysin. Immunoreaction was always cytoplasmic with differences in intensity, pattern of intracytoplasmic distribution and reaction type. Other cytokeratins, anti-S100 protein, anti-vimentin and anti-glial fibrillary acid protein were absent in canine normal MCs.
URI: http://hdl.handle.net/10553/73767
ISBN: 3-540-00374-6
Source: Merkel Cell: Structure-Development-Function-Cancerogenesis, p. 49-55, (2003)
Appears in Collections:Actas de congresos
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