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Title: Comparison of Three Histological Techniques for Fat Emboli Detection in Lung Cetacean's Tissue
Authors: Arregui Gil, Marina 
Fernández Rodríguez, Antonio Jesús 
Paz Sanchez, Yania 
Santana Del Pino, Ángelo 
Sacchini, Simona 
Sierra Pulpillo, Eva María 
Arbelo Hernández, Manuel Antonio 
Bernaldo De Quirós Miranda, Yara 
UNESCO Clasification: 240119 Zoología marina
310907 Patología
Keywords: Fat embolism
Issue Date: 2020
Journal: Scientific Reports 
Abstract: Fat embolism is the mechanical blockage of blood vessels by circulating fat particles. It is frequently related to traumas involving soft tissues and fat-containing bones. Different techniques have been used for decades to demonstrate histologically fat emboli, being the extremely toxic post-fixation with osmium tetroxide one of the most used techniques in the last decades. In the present study, the osmium tetroxide technique was compared qualitatively and quantitatively, for the first time, with chromic acid and Oil Red O frozen techniques for histological fat emboli detection in the lungs of eight sperm whales that died due to ship strikes. This was also the first time that chromic acid technique was tested in cetaceans. Results showed that the three techniques were valuable for the histological detection of fat embolism in cetaceans, even when tissues presented advanced autolysis and had been stored in formaldehyde for years. Although quantitative differences could not be established, the Oil Red O frozen technique showed the lowest quality for fat emboli staining. On the contrary, the chromic acid technique was proven to be a good alternative to osmium tetroxide due to its slightly lower toxicity, its equivalent or even superior capacity of fat emboli detection, and its significantly lower economic cost.
DOI: 10.1038/s41598-020-64821-8
Source: Scientific reports [EISSN 2045-2322], v. 10 (1), (Mayo 2020)
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