|Title:||Effect Of Nitrogen Supply On Photosynthesis And Carbonic-Anhydrase Activity In The Green Seaweed Ulva-Rigida (Chlorophyta)||Authors:||Jiménez del Río, Mj
García Reina, Guillermo
|UNESCO Clasification:||241707 Algología (ficología)||Keywords:||Fragile Ssp Tomentosoides
Marine-Environment, et al
|Issue Date:||1995||Journal:||Marine Biology||Abstract:||Photosynthesis rate and carbonic anhydrase (CA) activity have been studied in the green seaweed Ulva rigida C. Agardh (Chlorophyta) grown in seawater (SW) and SW supplemented with 40 mu M NH4Cl (N-SW). Higher growth and maximal O-2 evolution rates were observed in N-SW- than in SW-grown seaweeds. Western blot analysis of the total homogenates probed with antibodies raised against small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) showed crossreaction with a 15 kdalton polypeptide in both SW- and N-SW-grown plants, although the band was more intense in N-SW-grown plants. Carbonic anhydrase activities in the total homogenate and in the soluble protein fraction were higher in N-SW-grown plants. Although the pellets from both plants showed a considerable CA activity, the activity of CA in the thylakoid membranes was undetectable. The low nitrogen concentration is a major environmental factor that affects the level of RuBisCO and CA, and therefore CO2 assimilation in U. rigida.||URI:||http://hdl.handle.net/10553/73041||ISSN:||0025-3162||DOI:||10.1007/BF00349111||Source:||Marine Biology [ISSN 0025-3162], v. 123 (4), p. 687-691, (Octubre 1995)|
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