Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/71932
DC FieldValueLanguage
dc.contributor.authorJiménez del Río, M.en_US
dc.contributor.authorRamazanov, Z.en_US
dc.contributor.authorGarcía-Reina, G.en_US
dc.date.accessioned2020-05-04T08:59:20Z-
dc.date.available2020-05-04T08:59:20Z-
dc.date.issued1993en_US
dc.identifier.issn0032-0935en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/71932-
dc.description.abstractThe effect of nitrogen starvation on the NO3-dependent induction of nitrate reductase (NR) and nitrite reductases (NIR) has been investigated in the halophilic alga Dunaliella salina. When D. salina cells previously grown in a medium with NH4+as the only nitrogen source (NH4+-cells) were transferred into NO3-medium, NR was induced in the light. In contrast, when cells previously grown in N-free medium were transferred into a medium containing NO3-, NR was induced in light or in darkness. Nitrate-dependent NR induction, in darkness, in D. salina cells previously grown at a photon flux density of 500 umol · m-2 s-1 was observed after 4 h preculture in N-free medium, whilst in cells grown at 100 umol · m-2 s-1 NR induction was observed after 7-8 h. An inhibitor of mRNA synthesis (6-methylpurine) did not inhibit NO3--induced NR synthesis when the cells, previously grown in NH4+medium, were transferred into NO3-medium (at time 0 h) after 4-h-N starvation. However, when 6-methylpurine was added simultaneously with the transfer of the cells from NH4+to NO3-medium (at time 0 h), NO3-induced NR synthesis was completely inhibited. The activity of NIR decreased in N-starved cells and the addition of NO3-to those cells greatly stimulated NIR activity in the light. The ability to induce NR in darkness was observed when glutamine synthetase activity reached its maximal level during N starvation. Although cells grown in NO3-medium exhibited high NR activity, only 0.33% of the total NR was found in intact chloroplasts. We suggest that the ability, to induce NR in darkness is dependent on the level of N starvation, and that NR in D. salina is located in the cytosol. Light seems to play an indirect regulatory role on NO3-uptake and NR induction due to the expression of NR and NO3--transporter mRNAs.en_US
dc.languageengen_US
dc.relation.ispartofPlantaen_US
dc.sourcePlanta [ISSN 0032-0935], v. 192 (1), p. 40-45, (Octubre 1993)en_US
dc.subject241707 Algología (ficología)en_US
dc.subject.otherDunaliellaen_US
dc.subject.otherNitrate Reductase (Dark Induction, Intracellular Location)en_US
dc.subject.otherNitrite Reductaseen_US
dc.subject.otherChlamydomonas-Reinhardtiien_US
dc.subject.otherChlorella-Sorokinianaen_US
dc.subject.otherGlutamine-Synthetaseen_US
dc.subject.otherSelenastrum-Minutumen_US
dc.subject.otherNitrogenen_US
dc.subject.otherPhytochromeen_US
dc.titleDark induction of nitrate reductase in the halophilic alga Dunaliella salinaen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/BF00198690en_US
dc.identifier.scopus0028103050-
dc.identifier.isiA1994MG36300005-
dc.contributor.authorscopusid7102783473-
dc.contributor.authorscopusid6701327897-
dc.contributor.authorscopusid6603367985-
dc.identifier.eissn1432-2048-
dc.description.lastpage45en_US
dc.identifier.issue1-
dc.description.firstpage40en_US
dc.relation.volume192en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid4563121-
dc.contributor.daisngid1102389-
dc.contributor.daisngid1482151-
dc.description.numberofpages6en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:DELRIO, MJ-
dc.contributor.wosstandardWOS:RAMAZANOV, Z-
dc.contributor.wosstandardWOS:GARCIAREINA, G-
dc.date.coverdateOctubre 1993en_US
dc.identifier.ulpgces
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.fullNameGarcia-Blairsy Reina, Guillermo-
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