Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/69832
Title: Bone marrow-derived macrophage immortalization of LXR nuclear receptor-deficient cells
Authors: Ramón-Vázquez, Ana 
de la Rosa, Juan Vladimir 
Tabraue Tarbay, Carlos 
Castrillo, Antonio 
UNESCO Clasification: 32 Ciencias médicas
Keywords: Bone Marrow-Derived Macrophages
Gm-Csf
Immortalization
J2 Retrovirus
Lxr Nuclear Receptors
Issue Date: 2019
Journal: Methods in molecular biology (Clifton, N.J.) 
Abstract: Macrophages are professional phagocytic cells that play key roles in innate and adaptive immunity, metabolism, and tissue homeostasis. Lipid metabolism is tightly controlled at the transcriptional level, and one of the key players of this regulation in macrophages and other cell types is the LXR subfamily of nuclear receptors (LXRα and LXRβ). The use of LXR double knockout (LXR-DKO) macrophages in vitro has yielded extensive benefits in metabolism research, but this technique is hindered by primary macrophage cell expansion capability, which diminishes along terminal cell differentiation process. Here we detail a method to immortalize LXR double knockout bone marrow-derived macrophage cells at an early stage of differentiation, using a retroviral delivery of a combination of murine v-myc and v-raf oncogenes. This methodology enables the generation of autonomous self-renewing macrophages bearing an LXR-DKO genetic background, as a valuable tool for research in lipid metabolism and other LXR receptor-mediated effects.
URI: http://hdl.handle.net/10553/69832
ISSN: 1064-3745
DOI: 10.1007/978-1-4939-9130-3_6
Source: Methods in Molecular Biology [ISSN 1064-3745], v. 1951, p. 75-85
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

1
checked on May 2, 2021

Page view(s)

34
checked on May 3, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.