Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/6553
Title: Leptin receptors in human skeletal muscle
Authors: Guerra, Borja 
Santana, Alfredo 
Fuentes, Teresa 
Delgado Guerra,Safira 
Cabrera-Socorro, Alfredo
Dorado, Cecilia 
Calbet, Jose A. L. 
UNESCO Clasification: 241110 Fisiología del músculo
Keywords: Obesity
Adipose tissue
Hypothalamus
Perilipin
Issue Date: 2007
Journal: Journal of Applied Physiology 
Abstract: Human skeletal muscle expresses leptin receptor mRNA; however, it remains unknown whether leptin receptors (OB-R) are also expressed at the protein level. Fourteen healthy men (age = 33.1 +/- 2.0 yr, height = 175.9 +/- 1.7 cm, body mass = 81.2 +/- 3.8 kg, body fat = 22.5 +/- 1.9%; means +/- SE) participated in this investigation. The expression of OB-R protein was determined in skeletal muscle, subcutaneous adipose tissue, and hypothalamus using a polyclonal rabbit anti-human leptin receptor. Three bands with a molecular mass close to 170, 128, and 98 kDa were identified by Western blot with the anti-OB-R antibody. All three bands were identified in skeletal muscle: the 98-kDa and 170-kDa bands were detected in hypothalamus, and the 98-kDa and 128-kDa bands were detected in thigh subcutaneous adipose tissue. The 128-kDa isoform was not detected in four subjects, whereas in the rest its occurrence was fully explained by the presence of intermuscular adipose tissue, as demonstrated using an anti-perilipin A antibody. No relationship was observed between the basal concentration of leptin in serum and the 170-kDa band density. In conclusion, a long isoform of the leptin receptor with a molecular mass close to 170 kDa is expressed at the protein level in human skeletal muscle. The amount of 170-kDa protein appears to be independent of the basal concentration of leptin in serum.
URI: http://hdl.handle.net/10553/6553
ISSN: 8750-7587
DOI: 10.1152/japplphysiol.01313.2006
Source: Journal Of Applied Physiology [ISSN 8750-7587], v. 102 (5), p. 1786-1792
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