Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/6530
Title: Leptin receptor 170 kDa (OB-R170) protein expression is reduced in obese human skeletal muscle: a potential mechanism of leptin resistance
Authors: Fuentes, Teresa 
Ara, Ignacio
Guadalupe-Grau, Amelia
Larsen, S.
Stallknecht, B.
Olmedillas, Hugo
Santana, A. 
Helge, J.
Calbet, J. A. L. 
Guerra, B. 
UNESCO Clasification: 241110 Fisiología del músculo
Keywords: Leptin
Obesity
Muscle
Issue Date: 2010
Project: Influencia Del Ejercicio Físico Regular en la Expresión Proteica y Nivel de Fosforilación (Activación) de la Isoforma Larga Del Receptor de Leptina en Músculo Esquelético Humano. 
Journal: Experimental Physiology 
Abstract: To examine whether obesity-associated leptin resistance could be due to down-regulation of leptin receptors (OB-Rs) and/or up-regulation of suppressor of cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in skeletal muscle, which blunt janus kinase 2-dependent leptin signalling and signal transducer and activator of transcription 3 (STAT3) phosphorylation and reduce AMP-activated protein kinase (AMPK) and acetyl-coenzyme A carboxylase (ACC) phosphorylation. Deltoid and vastus lateralis muscle biopsies were obtained from 20 men: 10 non-obese control subjects (mean +/- s.d. age, 31 +/- 5 years; height, 184 +/- 9 cm; weight, 91 +/- 13 kg; and percentage body fat, 24.8 +/- 5.8%) and 10 obese (age, 30 +/- 7 years; height, 184 +/- 8 cm; weight, 115 +/- 8 kg; and percentage body fat, 34.9 +/- 5.1%). Skeletal muscle OB-R170 (OB-R long isoform) protein expression was 28 and 25% lower (both P < 0.05) in arm and leg muscles, respectively, of obese men compared with control subjects. In normal-weight subjects, SOCS3 protein expression, and STAT3, AMPKalpha and ACCbeta phosphorylation, were similar in the deltoid and vastus lateralis muscles. In obese subjects, the deltoid muscle had a greater amount of leptin receptors than the vastus lateralis, whilst SOCS3 protein expression was increased and basal STAT3, AMPKalpha and ACCbeta phosphorylation levels were reduced in the vastus lateralis compared with the deltoid muscle (all P < 0.05). In summary, skeletal muscle leptin receptors and leptin signalling are reduced in obesity, particularly in the leg muscles.
URI: http://hdl.handle.net/10553/6530
ISSN: 0958-0670
DOI: 10.1113/expphysiol.2009.049270
Source: Experimental Physiology[ISSN 0958-0670],v. 95 (1), p. 160-171
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