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Title: Skeletal muscle mitochondrial DNA content in exercising humans
Authors: Marcuello, A.
González-Alonso, J.
Calbet, José A.L. 
Lopez-Perez, M.
Diez-Sanchez, C.
Damsgaard, R.
UNESCO Clasification: 241106 Fisiología del ejercicio
Keywords: Human skeletal muscle
mtDNA content
Issue Date: 2005
Journal: Journal of Applied Physiology 
Abstract: Several weeks of intense endurance training enhances mitochondrial biogenesis in humans. Whether a single bout of exercise alters skeletal muscle mitochondrial DNA (mtDNA) content remains unexplored. Double-stranded mtDNA, estimated by slot-blot hybridization and real time PCR and expressed as mtDNA-to-nuclear DNA ratio (mtDNA/nDNA) was obtained from the vastus lateralis muscle of healthy human subjects to investigate whether skeletal muscle mtDNA changes during fatiguing and nonfatiguing prolonged moderate intensity [2.0-2.5 h; approximately 60% maximal oxygen consumption (Vo(2 max))] and short repeated high-intensity exercise (5-8 min; approximately 110% Vo(2 max)). In control resting and light exercise (2 h; approximately 25% Vo(2 max)) studies, mtDNA/nDNA did not change. Conversely, mtDNA/nDNA declined after prolonged fatiguing exercise (0.863 +/- 0.061 vs. 1.101 +/- 0.067 at baseline; n = 14; P = 0.005), remained lower after 24 h of recovery, and was restored after 1 wk. After nonfatiguing prolonged exercise, mtDNA/nDNA tended to decline (n = 10; P = 0.083) but was reduced after three repeated high-intensity exercise bouts (0.900 +/- 0.049 vs. 1.067 +/- 0.071 at baseline; n = 7; P = 0.013). Our findings indicate that prolonged and short repeated intense exercise can lead to significant reductions in human skeletal muscle mtDNA content, which might function as a signal stimulating mitochondrial biogenesis with exercise training.
ISSN: 8750-7587
DOI: 10.1152/japplphysiol.00289.2005
Source: Journal of Applied Physiology [ISSN 8750-7587] ,v. 99 (4), p. 1372-1377, (Octubre 2005)
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Skeletal muscle mitochondrial DNA content in exercising humans
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