Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/6512
Title: Skeletal muscle mitochondrial DNA content in exercising humans
Authors: Marcuello, A.
González-Alonso, J.
Calbet, José A.L. 
Lopez-Perez, M.
Diez-Sanchez, C.
Damsgaard, R.
UNESCO Clasification: 241106 Fisiología del ejercicio
Keywords: Human skeletal muscle
mtDNA content
Exercise
Issue Date: 2005
Journal: Journal of Applied Physiology 
Abstract: Several weeks of intense endurance training enhances mitochondrial biogenesis in humans. Whether a single bout of exercise alters skeletal muscle mitochondrial DNA (mtDNA) content remains unexplored. Double-stranded mtDNA, estimated by slot-blot hybridization and real time PCR and expressed as mtDNA-to-nuclear DNA ratio (mtDNA/nDNA) was obtained from the vastus lateralis muscle of healthy human subjects to investigate whether skeletal muscle mtDNA changes during fatiguing and nonfatiguing prolonged moderate intensity [2.0-2.5 h; approximately 60% maximal oxygen consumption (Vo(2 max))] and short repeated high-intensity exercise (5-8 min; approximately 110% Vo(2 max)). In control resting and light exercise (2 h; approximately 25% Vo(2 max)) studies, mtDNA/nDNA did not change. Conversely, mtDNA/nDNA declined after prolonged fatiguing exercise (0.863 +/- 0.061 vs. 1.101 +/- 0.067 at baseline; n = 14; P = 0.005), remained lower after 24 h of recovery, and was restored after 1 wk. After nonfatiguing prolonged exercise, mtDNA/nDNA tended to decline (n = 10; P = 0.083) but was reduced after three repeated high-intensity exercise bouts (0.900 +/- 0.049 vs. 1.067 +/- 0.071 at baseline; n = 7; P = 0.013). Our findings indicate that prolonged and short repeated intense exercise can lead to significant reductions in human skeletal muscle mtDNA content, which might function as a signal stimulating mitochondrial biogenesis with exercise training.
URI: http://hdl.handle.net/10553/6512
ISSN: 8750-7587
DOI: 10.1152/japplphysiol.00289.2005
Source: Journal of Applied Physiology [ISSN 8750-7587] ,v. 99 (4), p. 1372-1377, (Octubre 2005)
Appears in Collections:Artículos
Thumbnail
Skeletal muscle mitochondrial DNA content in exercising humans
Adobe PDF (264,53 kB)
Show full item record

SCOPUSTM   
Citations

39
checked on Dec 1, 2024

WEB OF SCIENCETM
Citations

40
checked on Nov 24, 2024

Page view(s)

90
checked on May 23, 2024

Download(s)

265
checked on May 23, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



This item is licensed under a Creative Commons License Creative Commons