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Title: Effects of different dietary selenium sources on growth performance, liver and muscle composition, antioxidant status, stress response and expression of related genes in gilthead seabream (Sparus aurata)
Authors: Mechlaoui, Marwa
Dominguez, David 
Robaina, Lidia 
Geraert, Pierre-Andre
Kaushik, Sadasivam
Mohamed Ibrahim Azam, Reda Saleh 
Briens, Mickael
Montero, Daniel 
Izquierdo, Marisol 
UNESCO Clasification: 310502 Piscicultura
Keywords: Trout Oncorhynchus-Mykiss
Hybrid Striped Bass
Oxidative Stress
2-Hydroxy-4-Methylselenobutanoic Acid, et al
Issue Date: 2019
Journal: Aquaculture 
Abstract: The present study aimed to determine the effects of dietary inclusion of selenium (Se) in the form of either inorganic Se (sodium selenite, NaSe) or organic Se (hydroxy-selenomethionine, OH-SeMet) on gilthead seabream (Sparus aurata). Triplicate groups of 150 fish each (6.2 +/- 0.04 g initial body weight) were distributed in 15 tanks and fed one of five experimental diets for 63 days. A control diet (C) without Se supplementation (0.8 mg Se/kg diet), was formulated and served as a basal diet for the other 4 experimental feeds, supplemented with either 0.2 or 0.5 mg Se/kg supplied in the form of NaSe or OH-SeMet. Fish growth performance, biochemical composition of liver and muscle and Se content, malondialdehyde (MDA) concentration in muscle and hepatopancreas, hepatocyte morphology and lysozyme activity in the serum were studied. Furthermore, expression of related hepatic genes, such as manganese superoxide dismutase (Mn-sod), catalase (cat), heat shock protein 70 (hsp70), and glutathione peroxidase (gpx) was also analyzed. After the trial, fish were exposed to an acute and chronic confinement stress. Blood samples for cortisol analysis were taken at 0 and 2 h after the acute stress and after 7 days of chronic stress.
ISSN: 0044-8486
DOI: 10.1016/j.aquaculture.2019.04.037
Source: Aquaculture [ISSN 0044-8486], v. 507, p. 251-259
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