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Title: Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status
Authors: Matiddi, Marco
deLucia, Giuseppe A.
Silvestri, Cecilia
Darmon, Gaelle
Tomas, Jesus
Pham, Christopher K.
Camedda, Andrea
Vandeperre, Frederic
Claro, Francoise
Kaska, Yakup
Kaberi, Helen
Revuelta, Ohiana
Piermarini, Raffaella
Daffina, Roberto
Pisapia, Marco
Genta, Daniela
Sozbilen, Dogan
Bradai, Mohamed N.
Rodriguez, Yasmina
Gambaiani, Delphine
Tsangaris, Catherine
Chaieb, Olfa
Moussier, Judicaelle
Liria Loza, Ana 
Miaud, Claude
INDICIT Consortium
UNESCO Clasification: 240113 Fisiología animal
Keywords: Caretta-Caretta
Debris Ingestion
Plastic ingestion
Sea turtle, et al
Issue Date: 2019
Project: Implementation of the second cycle of the Marine Strategy Framework Directive: achieving coherent, coordinated and consistent updates of the determinations of good environmental status, initial assesments and environmental targets 
Journal: Journal of Visualized Experiments 
Abstract: The following protocol is intended to respond to the requirements set by the European Union's Marine Strategy Framework Directives (MSFD) for the D10C3 Criteria reported in the Commission Decision (EU), related to the amount of litter ingested by marine animals. Standardized methodologies for extracting litter items ingested from dead sea turtles along with guidelines on data analysis are provided. The protocol starts with the collection of dead sea turtles and classification of samples according to the decomposition status. Turtle necropsy must be performed in authorized centers and the protocol described here explains the best procedure for gastrointestinal (GI) tract isolation. The three parts of the GI (esophagus, stomach, intestine) should be separated, opened lengthways and contents filtered using a 1 mm mesh sieve. The article describes the classification and quantification of ingested litter, classifying GI contents into seven different categories of marine litter and two categories of natural remains. The quantity of ingested litter should be reported as total dry mass (weight in grams, with two decimal places) and abundance (number of items). The protocol proposes two possible scenarios to achieve the Good Environmental Status (GES). First: "There should be less than X% of sea turtles having Y g or more plastic in the GI in samples of 50-100 dead turtles from each sub-region", where Y is the average weight of plastic ingested and X% is the percentage of sea turtles with more weight (in grams) of plastic than Y. The second one, which considers the food remain versus plastic as a proxy of individual health, is: "There should be less than X% of sea turtles having more weight of plastic (in grams) than food remains in the GI in samples of 50-100 dead turtles from each sub-region".
ISSN: 1940-087X
DOI: 10.3791/59466
Source: Jove-Journal Of Visualized Experiments [ISSN 1940-087X], n. 147, e59466
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