Please use this identifier to cite or link to this item:
Title: Hyperspectral Push-Broom Microscope Development and Characterization
Authors: Ortega Sarmiento, Samuel 
Guerra Hernández, Raúl Celestino 
Díaz Martín, María 
Fabelo Gómez, Himar Antonio 
López Suárez, Sebastián 
Marrero Callicó, Gustavo Iván 
Sarmiento Rodríguez, Roberto 
UNESCO Clasification: 3314 Tecnología médica
Keywords: Hyperspectral imaging
System analysis and design
Data acquisition
Image analysis, et al
Issue Date: 2019
Project: Identificación Hiperespectral de Tumores Cerebrales (Ithaca) 
Plataforma H2/Sw Distribuida Para El Procesamiento Inteligente de Información Sensorial Heterogenea en Aplicaciones de Supervisión de Grandes Espacios Naturales 
Journal: IEEE Access 
Abstract: Currently, the use of hyperspectral imaging (HSI) for the inspection of microscopic samples is an emerging trend in different fields. The use of push-broom hyperspectral (HS) cameras against other HSI technologies is motivated by their high spectral resolution and their capabilities to exploit spectral ranges beyond 1000 nm. Nevertheless, using push-broom cameras in miscroscopes imposes to perform an accurate spatial scanning of the sample to collect the HS data. In this manuscript, we present a methodology to correctly set-up a push-broom HS microscope to acquire high-quality HS images. Firstly, we describe a custom 3D printed mechanical system developed to perform the spatial scanning by producing a precise linear movement of the microscope stage. Then, we discuss how the dynamic range maximisation, the focusing, the alignment and the adequate speed determination affect the overall quality of the images. Finally, we present some examples of HS data showing the most common defects that usually appear when capturing HS images using a push-broom camera, and also a set of images acquired from real microscopic samples.
ISSN: 2169-3536
DOI: 10.1109/ACCESS.2019.2937729
Source: IEEE Access [ISSN 2169-3536], v. 7, p. 122473 - 122491
Appears in Collections:Artículos
Adobe PDF (7,9 MB)
Show full item record


checked on Oct 2, 2022

Page view(s)

checked on Dec 10, 2022


checked on Dec 10, 2022

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.