Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/56127
Title: Detection of Schistosoma mansoni-derived DNA in human urine samples by loop-mediated isothermal amplification (LAMP)
Authors: Fernández-Soto, Pedro
Gandasegui, Javier
Carranza Rodríguez, Cristina
Pérez Arellano, José Luis 
Crego-Vicente, Beatriz
García-Bernalt Diego, Juan
López-Abán, Julio
Vicente, Belén
Muro, Antonio
Editors: Mendlovic, Fela
UNESCO Clasification: 320505 Enfermedades infecciosas
Keywords: Schistosoma mansoni
Sub-Saharan Africa
LAMP assay
SmMIT-LAMP
Issue Date: 2019
Journal: PLoS ONE 
Abstract: Schistosoma mansoni is the main species causing hepatic and intestinal schistosomiasis in Sub-Saharan Africa, and it is the only species in South America. Adult stages of the parasite reside in the mesenteric venous plexus of infected hosts, and eggs are shed in feces. Collecting patient stool samples for S. mansoni diagnostic purposes is difficult in large-scale field trials. Urine samples would be an alternative approach for molecular S. mansoni detection since they have several advantages over stool samples, including better handling, management and storage. Additionally, loop-mediated isothermal amplification (LAMP) technology is a powerful molecular diagnostic tool for infectious diseases, particularly under field conditions in developing countries. The present study aimed to assess the effectiveness of our previously developed LAMP assay (SmMIT-LAMP) for S. mansoni-specific detection in clinical urine samples.
URI: http://hdl.handle.net/10553/56127
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0214125
Source: Plos One [ISSN 1932-6203], v. 14 (3), (Marzo 2019)
Appears in Collections:Artículos
Thumbnail
pdf
Adobe PDF (1,74 MB)
Show full item record

SCOPUSTM   
Citations

9
checked on Jul 25, 2021

WEB OF SCIENCETM
Citations

11
checked on Jul 25, 2021

Page view(s)

33
checked on Jun 26, 2021

Download(s)

33
checked on Jun 26, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.