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Title: Extraction optimization prior to HPLC analysis for phytoplankton pigments
Authors: Abaroa Pérez, B.
Vega-Moreno, D. 
Hernández Brito, José Joaquín 
UNESCO Clasification: 2301 química analítica
Keywords: Determining these Compounds
Main Technique
Issue Date: 2017
Journal: International journal of current research 
Abstract: Extracting marine phytoplankton pigments is a frequently-used analytical technique for determining the main groups present in samples. Determining these compounds (carotenoids and chlorophylls) is done mainly by high pressure liquid chromatography (HPLC), with visible UV detection and fluorescence, using a range of published methods. But the preliminary extraction required to determine them is a little-studied step that requires optimising. This paper analyses several factors that affect the extraction process, using ultra-sound microprobe as the main technique, validating the method with culture samples and real samples harvested at different depths.
ISSN: 0975-833X
Source: International journal of current research [ISSN 0975-833X ], v. 9 (9), p. 58083-58088
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