Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/55104
Title: Utility of Schistosoma bovis adult worm antigens for diagnosis of human schistosomiasis by enzyme-linked immunosorbent assay and electroimmunotransfer blot techniques
Authors: Pardo, J.
Carranza, C.
Turrientes, M. C.
Pérez Arellano, J. L. 
López Vélez, R.
Ramajo, V.
Muro, A.
Keywords: Polymerase-Chain-Reaction
Circulating Antigens
Cathodic Antigens
Mansoni
Haematobium, et al
Issue Date: 2004
Publisher: 1071-412X
Journal: Clinical and Diagnostic Laboratory Immunology 
Abstract: Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive.
URI: http://hdl.handle.net/10553/55104
ISSN: 1071-412X
DOI: 10.1128/CDLI.11.6.1165-1170.2004
Source: Clinical and Diagnostic Laboratory Immunology[ISSN 1071-412X],v. 11, p. 1165-1170
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

32
checked on Jun 13, 2021

WEB OF SCIENCETM
Citations

28
checked on Jun 13, 2021

Page view(s)

10
checked on Jun 14, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.