Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/55019
Title: Antioxidants Facilitate High-intensity Exercise IL-15 Expression in Skeletal Muscle
Authors: Pérez-López, Alberto
Martin-Rincon, Marcos 
Santana, Alfredo 
Pérez-Suárez, Ismael 
Dorado, Cecilia 
Calbet, Jose A.L. 
Morales-Alamo, David 
UNESCO Clasification: 241106 Fisiología del ejercicio
Keywords: Myokines
IL-15
Sprint exercise
Glycolysis
Oxidative stress
Issue Date: 2019
Project: Influencia Del Ejercicio Fisico, la Fio2 y El Estres Oxidativo en la Señalización Por Ampk en El Músculo Esquelético Humano. 
Desarrollo y Caracterización Molecular de Un Nuevo Modelo de Precondicionamiento Remoto 
Journal: International Journal of Sports Medicine 
Abstract: Interleukin (IL)-15 stimulates mitochondrial biogenesis, fat oxidation, glucose uptake and myogenesis in skeletal muscle. However, the mechanisms by which exercise triggers IL-15 expression remain to be elucidated in humans. This study aimed at determining whether high-intensity exercise and exercise-induced RONS stimulate IL-15/IL-15Rα expression and its signaling pathway (STAT3) in human skeletal muscle. Nine volunteers performed a 30-s Wingate test in normoxia and hypoxia (PIO2=75 mmHg), 2 h after placebo or antioxidant administration (α-lipoic acid, vitamin C and E) in a randomized double-blind design. Blood samples and muscle biopsies (vastus lateralis) were obtained before, immediately after, and 30 and 120 min post-exercise. Sprint exercise upregulated skeletal muscle IL-15 protein expression (ANOVA, P=0.05), an effect accentuated by antioxidant administration in hypoxia (ANOVA, P=0.022). In antioxidant conditions, the increased IL-15 expression at 120 min post-exercise (33%; P=0.017) was associated with the oxygen deficit caused by the sprint (r=–0.54; P=0.020); while, IL-15 and Tyr705-STAT3 AUCs were also related (r=0.50; P=0.036). Antioxidant administration promotes IL-15 protein expression in human skeletal muscle after sprint exercise, particularly in severe acute hypoxia. Therefore, during intense muscle contraction, a reduced PO2 and glycolytic rate, and possibly, an attenuated RONS generation may facilitate IL-15 production, accompanied by STAT3 activation, in a process that does not require AMPK phosphorylation.
URI: http://hdl.handle.net/10553/55019
ISSN: 0172-4622
DOI: 10.1055/a-0781-2527
Source: International Journal of Sports Medicine [ISSN 0172-4622], v. 40 (1), p. 16-22
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