Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54830
Title: Molecular characteristics of circulating tumor cells resemble the liver metastasis more closely than the primary tumor in metastatic colorectal cancer
Authors: Onstenk, Wendy
Sieuwerts, Anieta M.
Mostert, Bianca
Lalmahomed, Zarina
Bolt-de Vries, Joan B.
van Galen, Anne
Smid, Marcel
Kraan, Jaco
Van, Mai
de Weerd, Vanja
Ramírez-Moreno, Raquel 
Biermann, Katharina
Verhoef, Cornelis
Grünhagen, Dirk J.
Ijzermans, Jan N.M.
Gratama, Jan W.
Martens, John W.M.
Foekens, John A.
Sleijfer, Stefan
UNESCO Clasification: 320713 Oncología
Issue Date: 2016
Journal: Oncotarget 
Abstract: Background: CTCs are a promising alternative for metastatic tissue biopsies for use in precision medicine approaches. We investigated to what extent the molecular characteristics of circulating tumor cells (CTCs) resemble the liver metastasis and/or the primary tumor from patients with metastatic colorectal cancer (mCRC). Results: The CTC profiles were concordant with the liver metastasis in 17/23 patients (74%) and with the primary tumor in 13 patients (57%). The CTCs better resembled the liver metastasis in 13 patients (57%), and the primary tumor in five patients (22%). The strength of the correlations was not associated with clinical parameters. Nine genes (CDH1, CDH17, CDX1, CEACAM5, FABP1, FCGBP, IGFBP3, IGFBP4, and MAPT) displayed significant differential expressions, all of which were downregulated, in CTCs compared to the tissues in the 23 patients. Patients and Methods: Patients were retrospectively selected from a prospective study. Using the CellSearch System, CTCs were enumerated and isolated just prior to liver metastasectomy. A panel of 25 CTC-specific genes was measured by RT-qPCR in matching CTCs, primary tumors, and liver metastases. Spearman correlation coefficients were calculated and considered as continuous variables with r=1 representing absolute concordance and r=-1 representing absolute discordance. A cut-off of r>0.1 was applied in order to consider profiles to be concordant. Conclusions: In the majority of the patients, CTCs reflected the molecular characteristics of metastatic cells better than the primary tumors. Genes involved in cell adhesion and epithelial-to-mesenchymal transition were downregulated in the CTCs. Our results support the use of CTC characterization as a liquid biopsy for precision medicine.
URI: http://hdl.handle.net/10553/54830
ISSN: 1949-2553
DOI: 10.18632/oncotarget.10175
Source: Oncotarget [ISSN 1949-2553], v. 7, p. 59058-59069
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

36
checked on Jun 23, 2024

WEB OF SCIENCETM
Citations

29
checked on Jun 23, 2024

Page view(s)

61
checked on Feb 24, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.