Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54651
Title: Oxysterol-induced soluble endoglin release and its involvement in hypertension
Authors: Valbuena-Diez, Ana C.
Blanco, Francisco J.
Oujo, Barbara
Langa, Carmen
Gonzalez-Nuñez, María
Llano, Elena
Pendas, Alberto M.
Díaz Sarmiento, María Mercedes 
Castrillo, Antonio 
Lopez-Novoa, José M.
Bernabeu, Carmelo
UNESCO Clasification: 32 Ciencias médicas
320108 Ginecología
2302 Bioquímica
Keywords: Cell hypoxia
Hypertension
Preeclampsia
Pregnancy
Peptides
Issue Date: 2012
Journal: Circulation (New York, N.Y.) 
Abstract: Background-Ischemia in the placenta is considered the base of the pathogenesis of preeclampsia, a pregnancy-specific syndrome in which soluble endoglin (sEng) is a prognostic marker and plays a pathogenic role. Here, we investigated the effects of hypoxia and the downstream pathways in the release of sEng.Methods and Results-Under hypoxic conditions, the trophoblast-like cell line JAR showed an increase in sEng parallel to an elevated formation of reactive oxygen species. Because reactive oxygen species are related to the formation of oxysterols, we assessed the effect of 22-(R)-hydroxycholesterol, a natural ligand of the liver X receptor (LXR), and the LXR synthetic agonist T0901317. Treatment of JAR cells or human placental explants with 22-(R)-hydroxycholesterol or T0901317 resulted in a clear increase in sEng that was dependent on LXR. These LXR agonists induced an increased matrix metalloproteinase-14 expression and activity and a significant reduction of its endogenous inhibitor, tissue inhibitor of metalloproteinase-3. In addition, mice treated with LXR agonists underwent an increase in the plasma sEng levels, concomitant with an increase in arterial pressure. Moreover, transgenic mice overexpressing sEng displayed high blood pressure. Finally, administration of an endoglin peptide containing the consensus matrix metalloproteinase-14 cleavage site G-L prevented the oxysterol-dependent increase in arterial pressure and sEng levels in mice.Conclusions-These studies provide a clue to the involvement of the LXR pathway in sEng release and its pathogenic role in vascular disorders such as preeclampsia.
URI: http://hdl.handle.net/10553/54651
ISSN: 0009-7322
DOI: 10.1161/CIRCULATIONAHA.112.101261
Source: Circulation [ISSN 0009-7322], v. 126 (22), p. 2612-2624
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