Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/54586
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ruiz-García, Almudena | en_US |
dc.contributor.author | Monsalve, Eva | en_US |
dc.contributor.author | Novellasdemunt, Laura | en_US |
dc.contributor.author | Navarro-Sabaté, Áurea | en_US |
dc.contributor.author | Manzano, Anna | en_US |
dc.contributor.author | Rivero, Samuel | en_US |
dc.contributor.author | Castrillo Viguera, Antonio Jesús | en_US |
dc.contributor.author | Casado, Marta | en_US |
dc.contributor.author | Laborda, Jorge | en_US |
dc.contributor.author | Bartrons, Ramón | en_US |
dc.contributor.author | Díaz-Guerra, María José M. | en_US |
dc.date.accessioned | 2019-02-18T11:47:00Z | - |
dc.date.available | 2019-02-18T11:47:00Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/54586 | - |
dc.description.abstract | Macrophages activated through Toll receptor triggering increase the expression of the A(2A) and A(2B) adenosine receptors. In this study, we show that adenosine receptor activation enhances LPS-induced pfkfb3 expression, resulting in an increase of the key glycolytic allosteric regulator fructose 2,6-bisphosphate and the glycolytic flux. Using shRNA and differential expression of A(2A) and A(2B) receptors, we demonstrate that the A(2A) receptor mediates, in part, the induction of pfkfb3 by LPS, whereas the A(2B) receptor, with lower adenosine affinity, cooperates when high adenosine levels are present. pfkfb3 promoter sequence deletion analysis, site-directed mutagenesis, and inhibition by shRNAs demonstrated that HIF1 alpha is a key transcription factor driving pfkfb3 expression following macrophage activation by LPS, whereas synergic induction of pfkfb3 expression observed with the A(2) receptor agonists seems to depend on Sp1 activity. Furthermore, levels of phospho-AMP kinase also increase, arguing for increased PFKFB3 activity by phosphorylation in long term LPS-activated macrophages. Taken together, our results show that, in macrophages, endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. This process enhances the glycolytic flux and favors ATP generation helping to develop and maintain the long term defensive and reparative functions of the macrophages. | en_US |
dc.language | spa | en_US |
dc.publisher | 0021-9258 | |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.source | Journal of Biological Chemistry[ISSN 0021-9258],v. 286, p. 19247-19258 | en_US |
dc.subject.other | Nf-Kappa-B | |
dc.subject.other | Hypoxia-Inducible Factor-1-Alpha | |
dc.subject.other | Activated Protein-Kinase | |
dc.subject.other | Tumor-Necrosis-Factor | |
dc.subject.other | Up-Regulation | |
dc.subject.other | Transcription Factor | |
dc.subject.other | Factor Induction | |
dc.subject.other | Immune-Response | |
dc.subject.other | Nitric-Oxide | |
dc.subject.other | C/Ebp-Delta | |
dc.title | Cooperation of adenosine with macrophage toll-4 receptor agonists leads to increased glycolytic flux through the enhanced expression of PFKFB3 gene | en_US |
dc.type | info:eu-repo/semantics/Article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1074/jbc.M110.190298 | en_US |
dc.identifier.scopus | 79957587060 | - |
dc.identifier.isi | 000291027700007 | - |
dc.contributor.authorscopusid | 35077961800 | - |
dc.contributor.authorscopusid | 16039649600 | - |
dc.contributor.authorscopusid | 38661458800 | - |
dc.contributor.authorscopusid | 6507020659 | - |
dc.contributor.authorscopusid | 7003973260 | - |
dc.contributor.authorscopusid | 16040660200 | - |
dc.contributor.authorscopusid | 55445301000 | - |
dc.contributor.authorscopusid | 7103101615 | - |
dc.contributor.authorscopusid | 7003504698 | - |
dc.contributor.authorscopusid | 7007151916 | - |
dc.contributor.authorscopusid | 7004245830 | - |
dc.description.lastpage | 19258 | en_US |
dc.description.firstpage | 19247 | en_US |
dc.relation.volume | 286 | en_US |
dc.type2 | Artículo | en_US |
dc.contributor.daisngid | 5413547 | - |
dc.contributor.daisngid | 2280332 | - |
dc.contributor.daisngid | 4768916 | - |
dc.contributor.daisngid | 1750898 | - |
dc.contributor.daisngid | 1241005 | - |
dc.contributor.daisngid | 4837467 | - |
dc.contributor.daisngid | 225640 | - |
dc.contributor.daisngid | 466535 | - |
dc.contributor.daisngid | 408952 | - |
dc.contributor.daisngid | 153393 | - |
dc.contributor.daisngid | 1524431 | - |
dc.contributor.wosstandard | WOS:Ruiz-Garcia, A | - |
dc.contributor.wosstandard | WOS:Monsalve, E | - |
dc.contributor.wosstandard | WOS:Novellasdemunt, L | - |
dc.contributor.wosstandard | WOS:Navarro-Sabate, A | - |
dc.contributor.wosstandard | WOS:Manzano, A | - |
dc.contributor.wosstandard | WOS:Rivero, S | - |
dc.contributor.wosstandard | WOS:Castrillo, A | - |
dc.contributor.wosstandard | WOS:Casado, M | - |
dc.contributor.wosstandard | WOS:Laborda, J | - |
dc.contributor.wosstandard | WOS:Bartrons, R | - |
dc.contributor.wosstandard | WOS:Diaz-Guerra, MJM | - |
dc.date.coverdate | Junio 2011 | en_US |
dc.identifier.ulpgc | Sí | es |
dc.description.sjr | 3,495 | |
dc.description.jcr | 4,773 | |
dc.description.sjrq | Q1 | |
dc.description.jcrq | Q1 | |
dc.description.scie | SCIE | |
item.fulltext | Sin texto completo | - |
item.grantfulltext | none | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.orcid | 0000-0002-2057-2159 | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | Castrillo Viguera, Antonio Jesús | - |
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