Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54574
Title: Detection and discrimination of Loa loa, Mansonella perstans and Wuchereria bancrofti by PCR-RFLP and nested-PCR of ribosomal DNA ITS1 region
Authors: Jiménez, Maribel
González, Luis Miguel
Carranza, Cristina
Bailo, Begoña
Pérez-Ayala, Ana
Muro, Antonio
Pérez-Arellano, José Luis 
Gárate, Teresa
Keywords: Polymerase-Chain-Reaction
Human Occult Loiasis
Lymphatic Filariasis
Blood-Samples
Diagnosis, et al
Issue Date: 2011
Publisher: 0014-4894
Journal: Experimental parasitology 
Abstract: The ribosomal deoxyribonucleic acid (DNA) internal transcribed spacer region (ITS1) of two filarial nematodes, Loa loa and Mansonella perstans, was amplified and further sequenced to develop an species-specific polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol for the differentiation of both species from Wuchereria bancrofti, three filarial nematodes with blood circulating microfilariae. The ITS1-PCR product digested with the restriction endonuclease Ase I generated an specific diagnostic pattern for each of the three species. Moreover, three new specific nested-PCRs, targeting the ITS1 region, for differential detection of L boa, M. perstans and W. bancrofti were developed and used when the ITS1-PCR products were insufficient for the Ase I enzymatic digestion. These filarial species-specific molecular protocols were evaluated in forty blood samples from African adult immigrants attending in the Hospital Insular of Gran Canaria, Canarias, Spain. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/10553/54574
ISSN: 0014-4894
DOI: 10.1016/j.exppara.2010.06.019
Source: Experimental Parasitology[ISSN 0014-4894],v. 127, p. 282-286
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

19
checked on May 16, 2021

WEB OF SCIENCETM
Citations

22
checked on May 9, 2021

Page view(s)

14
checked on May 15, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.