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Title: Detection and discrimination of Loa loa, Mansonella perstans and Wuchereria bancrofti by PCR-RFLP and nested-PCR of ribosomal DNA ITS1 region
Authors: Jiménez, Maribel
González, Luis Miguel
Carranza Rodríguez, Cristina 
Bailo, Begoña
Pérez-Ayala, Ana
Muro, Antonio
Pérez-Arellano, José Luis 
Gárate, Teresa
UNESCO Clasification: 32 Ciencias médicas
320712 Parasitología
Keywords: Loa loa
Mansonella perstans
Wuchereria bancrofti
PCR–RFLP, et al
Issue Date: 2011
Journal: Experimental parasitology 
Abstract: The ribosomal deoxyribonucleic acid (DNA) internal transcribed spacer region (ITS1) of two filarial nematodes, Loa loa and Mansonella perstans, was amplified and further sequenced to develop an species-specific polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol for the differentiation of both species from Wuchereria bancrofti, three filarial nematodes with blood circulating microfilariae. The ITS1-PCR product digested with the restriction endonuclease Ase I generated an specific diagnostic pattern for each of the three species. Moreover, three new specific nested-PCRs, targeting the ITS1 region, for differential detection of L boa, M. perstans and W. bancrofti were developed and used when the ITS1-PCR products were insufficient for the Ase I enzymatic digestion. These filarial species-specific molecular protocols were evaluated in forty blood samples from African adult immigrants attending in the Hospital Insular of Gran Canaria, Canarias, Spain.
ISSN: 0014-4894
DOI: 10.1016/j.exppara.2010.06.019
Source: Experimental Parasitology [ISSN 0014-4894], v. 127(1), p. 282-286 (Enero 2011)
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