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Title: Arginase I induction by modified lipoproteins in macrophages: A peroxisome proliferator-activated receptor-γ/δ-mediated effect that links lipid metabolism and immunity
Authors: Gallardo-Soler, Alejandro
Gómez-Nieto, Carlos
Campo, María Luisa
Marathe, Chaitra
Tontonoz, Peter
Castrillo, Antonio 
Corraliza, Inés
Keywords: Marrow-Derived Macrophages
Nuclear Receptors, et al
Issue Date: 2008
Publisher: 0888-8809
Journal: Molecular Endocrinology 
Abstract: Macrophages are phagocytic cells that play essential roles in innate immunity and lipid homeostasis. The uptake of modified lipoproteins is an important early event in the development of atherosclerosis. We analyzed the ability of modified low-density lipoprotein (LDL) (oxidized and acetylated) to alter the expression and activity of arginases (ArgI and ArgII) in macrophages. We show that ArgI expression is potently induced by both oxidized and acetylated LDL in macrophages. We further show that this effect is mediated by peroxisome proliferator-activated receptors (PPAR). ArgI expression is highly responsive to agonists for PPAR gamma and PPAR delta but not PPAR alpha. Moreover, the induction of ArgI by both PPAR agonists and IL-4 is blocked in macrophages from PPAR gamma- and PPAR delta-deficient mice. Functionally, PPAR activity induces macrophage activation toward a more Th2 immune phenotype in a model of Leishmania major infection. We show that PPAR gamma and -delta ligands promote intracellular amastigote growth in infected macrophages, and this effect is dependent on both PPAR expression and Arg activity. Collectively, our results strongly suggest that ArgI is a key marker of the alternative program triggered by PPAR in macrophages.
ISSN: 0888-8809
DOI: 10.1210/me.2007-0525
Source: Molecular Endocrinology[ISSN 0888-8809],v. 22, p. 1394-1402
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