Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54414
Title: The arginase II gene is an anti-inflammatory target of liver X receptor in macrophages
Authors: Marathe, Chaitra
Bradley, Michelle N.
Hong, Cynthia
Lopez, Felix 
Ruiz De Galarreta, Carlos M.
Tontonoz, Peter
Castrillo, Antonio 
Keywords: Interferon Regulatory Factor-3
Nitric-Oxide Synthase
L-Arginine
Differential Regulation
Reciprocal Regulation, et al
Issue Date: 2006
Publisher: 0021-9258
Project: Saf2005-03270. Los Receptores Lxr y Su Papel en la Respuesta Inflamatoria, Inmunitaria y Apoptótica. 
Journal: Journal of Biological Chemistry 
Abstract: The liver X receptors (LXRs) are ligand-dependent transcription factors that have been implicated in lipid metabolism and inflammation. LXRs also inhibit the expression of inflammatory genes in macrophages, including inducible nitric oxide synthase (iNOS). Some of these actions are mediated through LXR antagonism of NF-kappa B activity. The potential for LXRs to positively regulate the expression of anti-inflammatory molecules, however, has not been explored. Here we show that the arginase II (ArgII) gene is a direct target for LXR regulation. ArgII catalyzes the conversion of L-arginine into L-ornithine and urea, leading to the synthesis of polyamines. Expression of ArgII is induced by LXR agonists in macrophage cell lines and primary murine macrophages in a receptor-dependent manner. The ArgII promoter contains a functional LXR response elements that mediates promoter induction by LXR/RXR (retinoid X receptor) in transfection assays. Since ArgII and iNOS utilize a common substrate, induction of ArgII expression has the potential to exert anti-inflammatory effects by shifting arginine metabolism toward polyamine synthesis at the expense of NO production. In support of this hypothesis, we demonstrate that forced expression of ArgII mimics the inhibitory effect of LXR activation on macrophage NO production. Furthermore, inhibition of arginase activity partially reverses the inhibitory effect of LXR agonists on NO production. These studies suggest that regulation of ArgII may contribute to the immunomodulatory effects of LXRs.
URI: http://hdl.handle.net/10553/54414
ISSN: 0021-9258
DOI: 10.1074/jbc.M605237200
Source: Journal of Biological Chemistry[ISSN 0021-9258],v. 281, p. 32197-32206
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

73
checked on Jun 20, 2021

WEB OF SCIENCETM
Citations

73
checked on Jun 20, 2021

Page view(s)

84
checked on Jun 21, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.