Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54400
DC FieldValueLanguage
dc.contributor.authorSandoval, N.en_US
dc.contributor.authorSiles-Lucas, M.en_US
dc.contributor.authorPérez-Arellano, J. L.en_US
dc.contributor.authorCarranza Rodríguez, Cristinaen_US
dc.contributor.authorPuente, S.en_US
dc.contributor.authorLópez-Abán, J.en_US
dc.contributor.authorMuro, A.en_US
dc.date.accessioned2019-02-18T10:34:28Z-
dc.date.available2019-02-18T10:34:28Z-
dc.date.issued2006en_US
dc.identifier.issn0031-1820en_US
dc.identifier.urihttp://hdl.handle.net/10553/54400-
dc.description.abstractCurrently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100%, sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.en_US
dc.languagespaen_US
dc.relation.ispartofParasitologyen_US
dc.sourceParasitology [ISSN 0031-1820], v. 133, p. 581-587en_US
dc.subject.otherPolymerase-Chain-Reactionen_US
dc.subject.otherMansoni Infectionsen_US
dc.subject.otherReaction Assayen_US
dc.subject.otherHaematobiumen_US
dc.subject.otherDiagnosisen_US
dc.subject.otherAntigensen_US
dc.subject.otherWateren_US
dc.subject.otherBovisen_US
dc.titleA new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samplesen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1017/S0031182006000898en_US
dc.identifier.scopus33750355586-
dc.identifier.isi000242237300007-
dc.contributor.authorscopusid57214572146-
dc.contributor.authorscopusid14523342000-
dc.contributor.authorscopusid15045746000-
dc.contributor.authorscopusid7005553929-
dc.contributor.authorscopusid6603949899-
dc.contributor.authorscopusid56219841000-
dc.contributor.authorscopusid12772685900-
dc.contributor.authorscopusid7006690116-
dc.description.lastpage587en_US
dc.description.firstpage581en_US
dc.relation.volume133en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid5763348-
dc.contributor.daisngid563367-
dc.contributor.daisngid445671-
dc.contributor.daisngid344572-
dc.contributor.daisngid20829289-
dc.contributor.daisngid1037139-
dc.contributor.daisngid409562-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Sandoval, N-
dc.contributor.wosstandardWOS:Siles-Lucas, M-
dc.contributor.wosstandardWOS:Perez-Arellano, JL-
dc.contributor.wosstandardWOS:Carranza, C-
dc.contributor.wosstandardWOS:Puente, S-
dc.contributor.wosstandardWOS:Lopez-Aban, J-
dc.contributor.wosstandardWOS:Muro, A-
dc.date.coverdateNoviembre 2006en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr1,786-
dc.description.jcrqQ2-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Didácticas Específicas-
crisitem.author.orcid0000-0002-2936-8242-
crisitem.author.orcid0000-0002-2768-0072-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNamePérez Arellano, José Luis-
crisitem.author.fullNameCarranza Rodríguez, Cristina-
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