Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/54400
Campo DC Valoridioma
dc.contributor.authorSandoval, N.en_US
dc.contributor.authorSiles-Lucas, M.en_US
dc.contributor.authorPérez-Arellano, J. L.en_US
dc.contributor.authorCarranza Rodríguez, Cristinaen_US
dc.contributor.authorPuente, S.en_US
dc.contributor.authorLópez-Abán, J.en_US
dc.contributor.authorMuro, A.en_US
dc.date.accessioned2019-02-18T10:34:28Z-
dc.date.available2019-02-18T10:34:28Z-
dc.date.issued2006en_US
dc.identifier.issn0031-1820en_US
dc.identifier.urihttp://hdl.handle.net/10553/54400-
dc.description.abstractCurrently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100%, sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.en_US
dc.languagespaen_US
dc.relation.ispartofParasitologyen_US
dc.sourceParasitology [ISSN 0031-1820], v. 133, p. 581-587en_US
dc.subject.otherPolymerase-Chain-Reactionen_US
dc.subject.otherMansoni Infectionsen_US
dc.subject.otherReaction Assayen_US
dc.subject.otherHaematobiumen_US
dc.subject.otherDiagnosisen_US
dc.subject.otherAntigensen_US
dc.subject.otherWateren_US
dc.subject.otherBovisen_US
dc.titleA new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samplesen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1017/S0031182006000898en_US
dc.identifier.scopus33750355586-
dc.identifier.isi000242237300007-
dc.contributor.authorscopusid57214572146-
dc.contributor.authorscopusid14523342000-
dc.contributor.authorscopusid15045746000-
dc.contributor.authorscopusid7005553929-
dc.contributor.authorscopusid6603949899-
dc.contributor.authorscopusid56219841000-
dc.contributor.authorscopusid12772685900-
dc.contributor.authorscopusid7006690116-
dc.description.lastpage587en_US
dc.description.firstpage581en_US
dc.relation.volume133en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid5763348-
dc.contributor.daisngid563367-
dc.contributor.daisngid445671-
dc.contributor.daisngid344572-
dc.contributor.daisngid20829289-
dc.contributor.daisngid1037139-
dc.contributor.daisngid409562-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Sandoval, N-
dc.contributor.wosstandardWOS:Siles-Lucas, M-
dc.contributor.wosstandardWOS:Perez-Arellano, JL-
dc.contributor.wosstandardWOS:Carranza, C-
dc.contributor.wosstandardWOS:Puente, S-
dc.contributor.wosstandardWOS:Lopez-Aban, J-
dc.contributor.wosstandardWOS:Muro, A-
dc.date.coverdateNoviembre 2006en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr1,786-
dc.description.jcrqQ2-
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Didácticas Específicas-
crisitem.author.orcid0000-0002-2936-8242-
crisitem.author.orcid0000-0002-2768-0072-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNamePérez Arellano, José Luis-
crisitem.author.fullNameCarranza Rodríguez, Cristina-
Colección:Artículos
Vista resumida

Citas SCOPUSTM   

121
actualizado el 24-mar-2024

Citas de WEB OF SCIENCETM
Citations

107
actualizado el 25-feb-2024

Visitas

55
actualizado el 10-mar-2024

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.