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Title: Increased rat alveolar macrophage expression of functional iNOS induced by a Dirofilaria immitis immunoglobulin superfamily protein
Authors: Andrade, M. Amparo
Siles-Lucas, Mar
Pérez Arellano, José Luis 
Barreto, Cristina Pou
Valladares, Basilio
Espinoza, Elsa
Muro, Antonio
Keywords: Nitric-Oxide Synthase
Chemically-Modified Tetracyclines
Wolbachia Bacteria
Filarial Nematodes, et al
Issue Date: 2005
Publisher: 1089-8603
Journal: Nitric Oxide - Biology and Chemistry 
Abstract: Dirofilaria immitis is a worldwide filarial nematode causing licartworm disease in dogs and cats. Several mosquito Species, Which are able to feed both on humans and animals, can transmit this parasite. Inflammatory progression of host tissues induced by parasites are mediated by several molecules, including nitric oxide (NO), which usually exerts deleterious effects on parasites and occasionally on the host. We analyze the in vitro effect of total D. immitis adult worm somatic antigens on naive rat alveolar macrophage NO production and further separation of parasite proteins to define specific D. immitis somatic molecules influencing host cell NO secretion. Additionally, we address the possible influence of Wolbachia spp. on the in vitro production of NO by macrophages. Our results demonstrate that D. immitis adult worm Soluble antigens are able to specifically induce NO production from host macrophages. Furthermore, we demonstrated that this effect is due to nematode antigens rather than to defined components (LPS and metabolic molecules) derived from its enclosymbiont, Wolbachia spp. In addition, we were able to isolate and identify one of the parasite specific components from the DiSo extract, denominated DiID35.3 and Putatively belonging to the Immunoglobulin Superfamily Protein (ISP) group, triggering NO release from macrophages in a dose-dependent and specific manner. (c) 2005 Elsevier Inc. All rights reserved.
ISSN: 1089-8603
DOI: 10.1016/j.niox.2005.06.003
Source: Nitric Oxide - Biology and Chemistry[ISSN 1089-8603],v. 13, p. 217-225
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