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Title: ABCG1 Is Required for Pulmonary B-1 B Cell and Natural Antibody Homeostasis
Authors: Baldan, Angel
Gonen, Ayelet
Choung, Christina
Que, Xuchu
Marquart, Tyler J.
Hernandez, Irene 
Bjorkhem, Ingemar
Ford, David A.
Witztum, Joseph L.
Tarling, Elizabeth J.
Keywords: Oxidation-Specific Epitopes
Binding Cassette Transporter
Atherosclerotic Lesions
Cholesterol Efflux, et al
Issue Date: 2014
Publisher: 0022-1767
Journal: Journal of Immunology 
Abstract: Many metabolic diseases, including atherosclerosis, type 2 diabetes, pulmonary alveolar proteinosis, and obesity, have a chronic inflammatory component involving both innate and adaptive immunity. Mice lacking the ATP-binding cassette transporter G1 (ABCG1) develop chronic inflammation in the lungs, which is associated with the lipid accumulation (cholesterol, cholesterol ester, and phospholipid) and cholesterol crystal deposition that are characteristic of atherosclerotic lesions and pulmonary alveolar proteinosis. In this article, we demonstrate that specific lipids, likely oxidized phospholipids and/or sterols, elicit a lung-specific immune response in Abcg1(-/-) mice. Loss of ABCG1 results in increased levels of specific oxysterols, phosphatidylcholines, and oxidized phospholipids, including 1-palmitoyl-2-(5'-oxovaleroyl)-sn-glycero-3-phosphocholine, in the lungs. Further, we identify a niche-specific increase in natural Ab (NAb)-secreting B-1 B cells in response to this lipid accumulation that is paralleled by increased titers of IgM, IgA, and IgG against oxidation-specific epitopes, such as those on oxidized low-density lipoprotein and malondialdehyde-modified low-density lipoprotein. Finally, we identify a cytokine/chemokine signature that is reflective of increased B cell activation, Ab secretion, and homing. Collectively, these data demonstrate that the accumulation of lipids in Abcg1(-/-) mice induces the specific expansion and localization of B-1 B cells, which secrete NAbs that may help to protect against the development of atherosclerosis. Indeed, despite chronic lipid accumulation and inflammation, hyperlipidemic mice lacking ABCG1 develop smaller atherosclerotic lesions compared with controls. These data also suggest that Abcg(-/-) mice may represent a new model in which to study the protective functions of B-1 B cells/NAbs and suggest novel targets for pharmacologic intervention and treatment of disease.
ISSN: 0022-1767
DOI: 10.4049/jimmunol.1400606
Source: Journal Of Immunology[ISSN 0022-1767],v. 193 (11), p. 5637-5648
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