Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53520
Title: Ceramide mediates tumor necrosis factor effects on P450-aromatase activity in cultured granulosa cells
Authors: Hernandez, I 
Quintana, J 
Santana, Pino 
Llanes, Luz 
Gallardo, Germán 
Gonzalez, Juan
Estevez, Francisco 
De Galarreta, Carlos Ruiz
Fanjul, Luisa F. 
UNESCO Clasification: Investigación
Keywords: Factor-Alpha
Sphingomyelin Turnover
Molecular-Cloning
Differentiation
Steroidogenesis
Receptor
Expression
Issue Date: 1995
Publisher: 0013-7227
Journal: Endocrinology (Philadelphia) 
Abstract: In [3H]serine-labelled granulosa cells treatment with TNF alpha (10 ng/ml) resulted in a transient decrease in cellular [3H]sphingomyelin and generation of [3H]ceramide that remained elevated 60 min later. In cells labelled with [methyl-14C]choline, TNF alpha induced a similar reduction in [14C]sphingomyelin content that was accompanied by a sustained elevation in [14C]phosphorylcholine levels. In FSH-primed cells, TNF alpha inhibited P450-AROM activity in a dose-dependent manner, an effect that was also observed in cells treated with bacterial sphingomyelinase (SMase 0.003-0.3 U/ml) or increasing concentrations (0.1-10 microM) of N-acetylsphingosine (C2-cer) a membrane-permeable analogue of ceramide. These results support the notion that sphingomyelin degradation to a bioeffector molecule ceramide, may be an early event involved in TNF alpha-induced signal transduction in granulosa cells.
URI: http://hdl.handle.net/10553/49179
ISSN: 0013-7227
DOI: 10.1210/endo.136.5.7720683
Source: Endocrinology [ISSN 0013-7227], v. 136 (5), p. 2345-2348
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