Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51920
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dc.contributor.authorHabib, Ibrahimen_US
dc.contributor.authorSmolarek, Dorotaen_US
dc.contributor.authorHattab, Claudeen_US
dc.contributor.authorGrodecka, Magdalenaen_US
dc.contributor.authorHassanzadeh-Ghassabeh, Gholamrezaen_US
dc.contributor.authorMuyldermans, Sergeen_US
dc.contributor.authorSagan, Sandrineen_US
dc.contributor.authorGutiérrez, Carlosen_US
dc.contributor.authorLaperche, Syriaen_US
dc.contributor.authorLe-Van-Kim, Carolineen_US
dc.contributor.authorAronovicz, Yves Colinen_US
dc.contributor.authorWasniowska, Kazimieraen_US
dc.contributor.authorGangnard, Stephaneen_US
dc.contributor.authorBertrand, Olivieren_US
dc.contributor.authorColin Aronovicz, Yvesen_US
dc.contributor.otherGutierrez, Carlos-
dc.contributor.otherSAGAN, Sandrine-
dc.contributor.othercolin, yves-
dc.contributor.otherMuyldermans, Serge-
dc.contributor.otherWasniowska, Kazimiera-
dc.contributor.otherle van kim, caroline-
dc.date.accessioned2018-11-25T05:25:42Z-
dc.date.available2018-11-25T05:25:42Z-
dc.date.issued2013en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10553/51920-
dc.description.abstractThe preparation of a VHH (nanobody) named IH4 that recognizes human glycophorin A (GPA) is described. IH4 was isolated by screening a library prepared from the lymphocytes of a dromedary immunized by human blood transfusion. Phage display and panning against GPA as the immobilized antigen allowed isolating this VHH. IH4, representing 67% of the retrieved VHH sequences, was expressed as a soluble correctly folded protein in SHuffle Escherichia coli cells, routinely yielding approximately 100 mg/L fermentation medium. Because IH4 recognizes GPA independently of the blood group antigens, it recognizes red cells of all humans with the possible exception of those with some extremely rare genetic background. The targeted linear epitope comprises the GPA Y52PPE55 sequence. Based on surface plasmon resonance results, the dissociation constant of the IH4-GPA equilibrium is 33 nM. IH4 is a stable protein with a transition melting temperature of 75.8 C (measured by differential scanning calorimetry). As proof of concept, we fused HIV p24 to IH4 and used the purified construct expressed in E. coli to show that IH4 was amenable to the preparation of autologous erythrocyte agglutination reagents: reconstituted blood prepared with serum from an HIV-positive patient was readily agglutinated by the addition of the bifunctional reagent.en_US
dc.languageengen_US
dc.relation.ispartofAnalytical biochemistry (Print)en_US
dc.sourceAnalytical Biochemistry [ISSN 0003-2697], v. 438 (1), p. 82-89en_US
dc.subject.otherHiv-1 Infectionen_US
dc.subject.otherChagas-Diseaseen_US
dc.subject.otherWhole-Blooden_US
dc.subject.otherAntibodiesen_US
dc.subject.otherDiagnosisen_US
dc.subject.otherProteinsen_US
dc.subject.otherNeurocysticercosisen_US
dc.subject.otherEliminationen_US
dc.subject.otherPerformanceen_US
dc.subject.otherAntigensen_US
dc.titleVHH (nanobody) directed against human glycophorin A: A tool for autologous red cell agglutination assaysen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.ab.2013.03.020en_US
dc.identifier.scopus84877064426-
dc.identifier.isi000319845200014-
dcterms.isPartOfAnalytical Biochemistry-
dcterms.sourceAnalytical Biochemistry[ISSN 0003-2697],v. 438 (1), p. 82-89-
dc.contributor.authorscopusid55674181200-
dc.contributor.authorscopusid23566823200-
dc.contributor.authorscopusid6602293547-
dc.contributor.authorscopusid16068372800-
dc.contributor.authorscopusid25645875200-
dc.contributor.authorscopusid7005810441-
dc.contributor.authorscopusid56189959700-
dc.contributor.authorscopusid7202545218-
dc.contributor.authorscopusid7003541857-
dc.contributor.authorscopusid7003841661-
dc.contributor.authorscopusid56000648000-
dc.contributor.authorscopusid55906233400-
dc.contributor.authorscopusid23987485300-
dc.contributor.authorscopusid56531546900-
dc.description.lastpage89en_US
dc.identifier.issue1-
dc.description.firstpage82en_US
dc.relation.volume438en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000319845200014-
dc.contributor.daisngid342829-
dc.contributor.daisngid2496502-
dc.contributor.daisngid2360218-
dc.contributor.daisngid2508043-
dc.contributor.daisngid1654264-
dc.contributor.daisngid90348-
dc.contributor.daisngid310875-
dc.contributor.daisngid475608-
dc.contributor.daisngid178147-
dc.contributor.daisngid276658-
dc.contributor.daisngid243062-
dc.contributor.daisngid853720-
dc.contributor.daisngid2487683-
dc.contributor.daisngid34787553-
dc.contributor.daisngid164048-
dc.identifier.investigatorRIDE-2989-2012-
dc.identifier.investigatorRIDI-8132-2013-
dc.identifier.investigatorRIDO-1910-2017-
dc.identifier.investigatorRIDC-6418-2016-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Habib, I-
dc.contributor.wosstandardWOS:Smolarek, D-
dc.contributor.wosstandardWOS:Hattab, C-
dc.contributor.wosstandardWOS:Grodecka, M-
dc.contributor.wosstandardWOS:Hassanzadeh-Ghassabeh, G-
dc.contributor.wosstandardWOS:Muyldermans, S-
dc.contributor.wosstandardWOS:Sagan, S-
dc.contributor.wosstandardWOS:Gutierrez, C-
dc.contributor.wosstandardWOS:Laperche, S-
dc.contributor.wosstandardWOS:Le-Van-Kim, C-
dc.contributor.wosstandardWOS:Colin Aronovicz, Y-
dc.contributor.wosstandardWOS:Wasniowska, K-
dc.contributor.wosstandardWOS:Gangnard, S-
dc.contributor.wosstandardWOS:Bertrand, O-
dc.date.coverdateJulio 2013en_US
dc.identifier.ulpgcen_US
dc.description.sjr0,854
dc.description.jcr2,305
dc.description.sjrqQ2
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Medicina Veterinaria e Investigación Terapéutica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0003-0764-7408-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameGutiérrez Cabrera,Carlos Javier-
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