Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51672
Title: Temperature-programmed high performance liquid chromatography separation of mono- and divinyl chlorophyll forms from marine phytoplankton
Authors: Van Lenning, K.
Garrido, J. L.
Aristegui, J. 
Zapata, M.
UNESCO Clasification: 241705 Biología marina
Keywords: Subtropical North-Atlantic
Prochlorococcus-Marinus
Hplc-Analysis
Pigments
Carotenoids, et al
Issue Date: 1995
Publisher: 0009-5893
Journal: Chromatographia (Wiesbaden) 
Abstract: Divinyl chlorophyll a (a2) and divinyl chlorophyll b (b2) are chemotaxonomic marker pigments for the marine prochlorophytes, and can be used to study their distribution in marine samples. In this paper we report a baseline resolution of mono- and divinyl forms of chlorophylls a and b employing polymeric ODS stationary phases at sub-ambient temperatures. The simultaneous resolution of mono- and divinyl forms of chlorophylls a and b, chlorophylls c1, c2, c3, Mg 3,8-divinylphaeoporphyrin a5 monomethyl ester (MgDVP), and phytol-substituted chlorophylls c was achieved when a temperature step gradient was employed during the analysis. An example is given of the utility of the protocol in oceanic field samples.
URI: http://hdl.handle.net/10553/51672
ISSN: 0009-5893
DOI: 10.1007/BF02688083
Source: Chromatographia [ISSN 0009-5893], v. 41, p. 539-543
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