Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51552
Título: Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified in vitro produced goat embryos
Autores/as: Rodríguez-Dorta, N.
Cognié, Y.
González, F.
Poulin, N.
Guignot, F.
Touzé, J. L.
Baril, G.
Cabrera, F. 
Álamo, D.
Batista, M. 
Gracia, A. 
Mermillod, P.
Palabras clave: Amino-Acids
Sheep
Culture
Vivo
Maturation, et al.
Fecha de publicación: 2007
Editor/a: 0093-691X
Publicación seriada: Theriogenology 
Resumen: This study evaluates the effect of coculture with goat oviduct epithelial cells (GOEC) on the pregnancy rate, embryo survival rate and offspring development after direct transfer of vitrified/thawed caprine in vitro produced (IVP) embryos. Oocytes were recovered from slaughterhouse goat ovaries, matured and inseminated with frozen/thawed capacitated semen, and presumptive zygotes were randomly cultured in synthetic oviduct fluid (SOF) (n = 352) or GOEC (n = 314). The percentage of cleaved embryos reaching the blastocyst stage was 28% and 20% in SOF and GOEC, respectively (P < 0.05). Overall, 26 blastocysts of SOF were transferred freshly in pairs to recipient goats, whereas 58 of SOF and 36 of GOEC were vitrified and transferred directly in pairs to recipient goats after thawing without removal of cryoprotectants or morphological evaluation. The kidding rate was 92% for SOF fresh, 14% for SOF vitrified (P < 0.001) and 56% for GOEC vitrified (P < 0.05); the difference was also significant between vitrified groups (P < 0.01). The embryo survival rate was 62% for SOF fresh, 9% for SOF vitrified (P < 0.001) and 33% for GOEC vitrified (P < 0.05) with a significant difference between vitrified groups (P < 0.01). The results showed that the coculture of IVP goat embryos with GOEC significantly improves the pregnancy and embryo survival rates and leads to the birth of healthy offspring. However, further research using more defined GOEC coculture is required to confirm its capacity to increase the success rate of IVP embryo technology in goat. (C) 2007 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/10553/51552
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2007.07.004
Fuente: Theriogenology[ISSN 0093-691X],v. 68, p. 908-913
Colección:Artículos
Vista completa

Citas SCOPUSTM   

33
actualizado el 24-mar-2024

Citas de WEB OF SCIENCETM
Citations

28
actualizado el 25-feb-2024

Visitas

37
actualizado el 20-may-2023

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.