Please use this identifier to cite or link to this item:
Title: Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified in vitro produced goat embryos
Authors: Rodríguez-Dorta, N.
Cognié, Y.
González, F.
Poulin, N.
Guignot, F.
Touzé, J. L.
Baril, G.
Cabrera, F. 
Álamo, D.
Batista, M. 
Gracia, A. 
Mermillod, P.
Keywords: Amino-Acids
Maturation, et al
Issue Date: 2007
Publisher: 0093-691X
Journal: Theriogenology 
Abstract: This study evaluates the effect of coculture with goat oviduct epithelial cells (GOEC) on the pregnancy rate, embryo survival rate and offspring development after direct transfer of vitrified/thawed caprine in vitro produced (IVP) embryos. Oocytes were recovered from slaughterhouse goat ovaries, matured and inseminated with frozen/thawed capacitated semen, and presumptive zygotes were randomly cultured in synthetic oviduct fluid (SOF) (n = 352) or GOEC (n = 314). The percentage of cleaved embryos reaching the blastocyst stage was 28% and 20% in SOF and GOEC, respectively (P < 0.05). Overall, 26 blastocysts of SOF were transferred freshly in pairs to recipient goats, whereas 58 of SOF and 36 of GOEC were vitrified and transferred directly in pairs to recipient goats after thawing without removal of cryoprotectants or morphological evaluation. The kidding rate was 92% for SOF fresh, 14% for SOF vitrified (P < 0.001) and 56% for GOEC vitrified (P < 0.05); the difference was also significant between vitrified groups (P < 0.01). The embryo survival rate was 62% for SOF fresh, 9% for SOF vitrified (P < 0.001) and 33% for GOEC vitrified (P < 0.05) with a significant difference between vitrified groups (P < 0.01). The results showed that the coculture of IVP goat embryos with GOEC significantly improves the pregnancy and embryo survival rates and leads to the birth of healthy offspring. However, further research using more defined GOEC coculture is required to confirm its capacity to increase the success rate of IVP embryo technology in goat. (C) 2007 Elsevier Inc. All rights reserved.
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2007.07.004
Source: Theriogenology[ISSN 0093-691X],v. 68, p. 908-913
Appears in Collections:Artículos
Show full item record


checked on Mar 26, 2023


checked on Oct 2, 2022

Page view(s)

checked on Mar 25, 2023

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.