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Title: Characterization of a mycoplasma agalactiae strain, candidate to an attenuated vaccine
Authors: Assunção, Patricia
Delafe, Christian
Ramírez, Ana S. 
Sarradell, Javier E.
Poveda, Jose B. 
Keywords: Immunogenicity
Issue Date: 2004
Publisher: 0971-2119
Journal: Journal of Applied Animal Research 
Abstract: The aim of this work was to study the stability of a M. agalactiae attenuated strain after lyophilization and its characterization by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Immunoblot against homologous antisera showed that the attenuated M. agalactiae strain had 2 characteristic antigenic bands of 32 and 21 kDa. The assays related to strain lyophilization showed that the CFUs were decreased lesser than 2log(10) after 1 year storage at 4C with the exception of one vial, which lost all its viability. The subcutaneous inoculation dose (10(5) CFU), did not produce any symptom or sign of disease during the 45 days of the experiment. However, a local reaction was observed on the day after the inoculation. At necropsy, no macroscopic lesions were found which related to contagious agalactia or other disease. These results suggest that this attenuated strain has good possibilities as a live attenuated vaccine.
ISSN: 0971-2119
DOI: 10.1080/09712119.2004.9706495
Source: Journal of Applied Animal Research[ISSN 0971-2119],v. 26, p. 1-5
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