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Title: The detection of Cryptosporidium spp. in the feces of a preschool population: a comparison of 5 staining methods
Authors: Martín Sánchez, A. M. 
Rodríguez Hernández, J.
Canut Blasco, A.
Dovigo Prieto, C.
UNESCO Clasification: 32 Ciencias médicas
3205 Medicina interna
Issue Date: 1993
Journal: Revista clínica española (Ed. impresa) 
Abstract: The usefulness of five dying techniques to detect oocysts of Cryptosporidium spp in stools is evaluated in a healthy children preschool population, taking from a smear of the sediment obtained in the formalin-ether concentration technique (Ritchie's test). 10 positive stool samples were detected of a total of 115 samples (8.7%). Dying with Lugol the sediment of Ritchie's test only detected 40% of positive samples. Modified Ziehl-Neelsen dye, auramine dye and indirect immunofluorescence dye with monoclonal antibodies, yielded an excellent diagnostic correlation (each of them detected 90% of the positive samples). Indirect immunofluorescence dye with specific human antibodies detected one case more of parasites than the rest of dyes, is not expensive and easy to perform.
ISSN: 0014-2565
Source: Revista clínica española[ISSN 0014-2565],v. 192, p. 63-66
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