|Title:||Androgen regulation of progestin biosynthetic enzymes in FSH-treated rat granulosa cells in vitro||Authors:||Welsh, T. H.
Jones, P. B.C.
De Galarreta, C. M.Ruiz
Fanjul, L. F.
Hsueh, A. J.W.
|UNESCO Clasification:||32 Ciencias médicas
320102 Genética clínica
|Issue Date:||1982||Journal:||Steroids||Abstract:||The influence of androgens on the FSH modulation of progestin biosynthetic enzymes was studied . Granulosa cells obtained from immature, hypophysectomized, estrogen-treated rats were cultured for 3 days in a serum-free medium containing FSH (20 ng/ml) with or without increasing concentrations (10−9−10−6 M) of 17β-hydroxy-5α-androstan-3-one (dihydrotestosterone; DHT), 5α-androstane-3α, 17β-diol (3α-diol), or the synthetic androgen 17β-hydroxy-17-methyl-4,9,11-estratrien-3-one (methyltrienolone; R1881). FSH treatment increased progesterone and 20α-hydroxy-4-pregnen-3-one(20α-OH-P) production by 10.2- and 11-fold, respectively. Concurrent androgen treatment augmented FSH-stimulated progesterone and 20α-OH-P production in a dose-related manner (R1881 > 3α-diol > DHT). In the presence of an inhibitor of 3β-hydroxysteroid dehydrogenase (3β-HSD), the FSH-stimulated pregnenolone (3β-hydroxy-5-pregnen-20-one) production (a 20-fold increase) was further enhanced by co-treatment with R1881, 3α-diol or DHT. Furthermore, FSH treatment increased 4.4-fold the activity of 3β-HSD, which converts pregnenolone to progesterone. This stimulatory action of FSH was further augmented by concurrent androgen treatment. In contrast, androgen treatment did not affect FSH-stimulated activity of a progesterone breakdown enzyme, 20α-hydroxysteroid dehydrogenase(20α-HSD). These results demonstrate that the augmenting effect of androgens upon FSH-stimulated progesterone biosynthesis is not due to changes in the conversion of progesterone to 20α-OH-P, but involves an enhancing action upon 3β-HSDΔ5, Δ4-isomerase complexes and additional enzymes prior to pregnenolone biosynthesis.||URI:||http://hdl.handle.net/10553/50833||ISSN:||0039-128X||DOI:||10.1016/0039-128X(82)90010-1||Source:||Steroids[ISSN 0039-128X],v. 40, p. 691-700 (Diciembre 1982)|
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