Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50585
DC FieldValueLanguage
dc.contributor.authorFanjul, L. F.en_US
dc.contributor.authorQuintana Aguiar, José Martínen_US
dc.contributor.authorGonzalez, J.en_US
dc.contributor.authorSantana Delgado, María Del Pinoen_US
dc.contributor.authorRuiz De Galarreta Hernandez,C. Manuelen_US
dc.contributor.authorEstévez Rosas, Francisco Jesúsen_US
dc.contributor.otherQuintana, Jose-
dc.contributor.otherEstevez, Francisco-
dc.date.accessioned2018-11-24T17:12:02Z-
dc.date.available2018-11-24T17:12:02Z-
dc.date.issued1992en_US
dc.identifier.issn0022-0795en_US
dc.identifier.urihttp://hdl.handle.net/10553/50585-
dc.description.abstractThe in-vivo regulatory effect of androgens on steroidogenesis was investigated. Adult (2 to 3 months old) hypophysectomized rats were treated intratesticularly with increasing doses of 5-alpha-dihydrotestosterone (DHT; 10-200-mu-g/100 g body weight) or vehicle (50-mu-l dimethyl sulphoxide; DMSO) in the contralateral testis. Intratesticular testosterone concentrations were extremely low in hypophysectomized rats 15-20 days after surgery. Treatment with DHT caused a dose-dependent inhibition of testicular 3-beta-hydroxysteroid dehydrogenase/DELTA-(5-4) isomerase (3-beta-HSD) 2 h later, and this effect was apparent at the dose of 20-mu-g/100 g body weight (P < 0.01). The inhibitory effect of 3-beta-HSD was not due to a possible interference of DHT in the enzyme assay, since various concentrations of the androgen (0.1-100-mu-mol/l) were ineffective as inhibitors of 3-beta-HSD. The highest dose of DHT used in this study (200-mu-g/100 g body weight) resulted in a rapid (1-2 h) and transient (4-6 h) inhibition (approximately 80%) of 3-beta-HSD activity. Pretreatment of rats with the antiandrogen cyproterone acetate (5 mg/rat) or the protein synthesis inhibitor cycloheximide (10 mg/rat) did not affect the enzyme activity of testes injected with DMSO, but counteracted the inhibitory effect of DHT on 3-beta-HSD activity in the contralateral testis. The results presented suggest that the inhibitory effect of the non-aromatizable androgen DHT is receptor-mediated and involves the synthesis of a factor(s) that modulates 3-beta-HSD activity.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Endocrinologyen_US
dc.sourceJournal Of Endocrinology[ISSN 0022-0795],v. 133 (2), p. 237-243en_US
dc.subject32 Ciencias médicasen_US
dc.subject320502 Endocrinologíaen_US
dc.subject.otherCorticotropin-Releasing Factoren_US
dc.subject.otherHuman Chorionic-Gonadotropinen_US
dc.subject.otherLeydig-Cell-Functionen_US
dc.subject.otherLuteinizing-Hormoneen_US
dc.subject.otherAndrogen Biosynthesisen_US
dc.subject.otherMessenger-Rnaen_US
dc.subject.otherGrowth-Factoren_US
dc.subject.otherTestisen_US
dc.subject.otherTestosteroneen_US
dc.subject.otherLocalizationen_US
dc.titleTesticular 3β-hydroxysteroid dehydrogenase/Δ<sup>5-4</sup> isomerase in the hypophysectomized rat: Effect of treatment with 5α-dihydrotestosteroneen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1677/joe.0.1330237en_US
dc.identifier.scopus0026566785-
dc.identifier.isiWOS:A1992HV66200011-
dcterms.isPartOfJournal Of Endocrinology-
dcterms.sourceJournal Of Endocrinology[ISSN 0022-0795],v. 133 (2), p. 237-243-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid8681043500-
dc.contributor.authorscopusid57198495832-
dc.contributor.authorscopusid7003526778-
dc.contributor.authorscopusid7003810011-
dc.contributor.authorscopusid7003806034-
dc.description.lastpage243en_US
dc.identifier.issue2-
dc.description.firstpage237en_US
dc.relation.volume133en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:A1992HV66200011-
dc.contributor.daisngid1127140-
dc.contributor.daisngid128315-
dc.contributor.daisngid13367402-
dc.contributor.daisngid329218-
dc.contributor.daisngid31449715-
dc.contributor.daisngid384944-
dc.contributor.daisngid1664323-
dc.identifier.investigatorRIDK-5709-2014-
dc.identifier.investigatorRIDK-5125-2014-
dc.description.numberofpages7en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:FANJUL, LF-
dc.contributor.wosstandardWOS:QUINTANA, J-
dc.contributor.wosstandardWOS:GONZALEZ, J-
dc.contributor.wosstandardWOS:SANTANA, P-
dc.contributor.wosstandardWOS:ESTEVEZ, F-
dc.contributor.wosstandardWOS:DEGALARRETA, CMR-
dc.date.coverdateMayo 1992en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.orcid0009-0000-1982-055X-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.orcid0000-0002-4093-2692-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
crisitem.author.fullNameQuintana Aguiar, José Martín-
crisitem.author.fullNameSantana Delgado, María Del Pino-
crisitem.author.fullNameRuiz De Galarreta Hernandez,C. Manuel-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
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