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Title: Design, synthesis, and biological evaluation of hybrid molecules containing alpha-methylene-gamma-butyrolactones and polypyrrole minor groove binders
Authors: Baraldi, Pier Giovanni
Del Carmen Nunez, Maria
Tabrizi, Mojgan Aghazadeh
De Clercq, Erik
Balzarini, Jan
Bermejo, Jaime
Estévez, Francisco 
Romagnoli, Romeo
UNESCO Clasification: 3201 Ciencias clínicas
320104 Patología clínica
Keywords: Lactone Tumor Inhibitors
Antitumor Agents
Issue Date: 2004
Journal: Journal of Medicinal Chemistry 
Abstract: We have synthesized and evaluated a series of hybrids of polypyrrole minor groove binders structurally related to the natural antitumor agent distamycin A, and α-methylene-γ-butyrolactones with methyl, phenyl, and 4-substituted phenyl groups at the lactone C(γ) position, denoted 5-17, for in vitro cytotoxic activity against a variety of cancer cell lines. The apoptotic and cytotoxic activities against several tumor cell lines are reported and discussed in terms of their structural differences in relation to both the number of N-methylpyrrole rings and the type of the alkylating unit tethered to the oligopeptidic frame. It may be noted that in general, and especially for 11, 12, and 17, the cytotoxicity of the hybrids was much greater than that of the α-methylene-γ-butyrolactone units 24a-g alone. Using the human leukemia cell line HL-60, we have tested the effects of a selected series of compounds on programmed cell death (apoptosis). The results clearly indicate that 11, 12, and 17, but not 9, are able to induce apoptosis as demonstrated from (i) identification of nuclear changes associated with apoptosis using fluorescence microscopy and (ii) by DNA laddering on agarose gel electrophoresis. Compound 12 was the most potent, especially after a short incubation period. It induced extensive hydrolysis of poly ADP-ribose polymerase (PARP), considered to be a hallmark of apoptosis, which plays a critical role in chromatin architecture and DNA metabolism.
ISSN: 0022-2623
DOI: 10.1021/jm031104y
Source: Journal Of Medicinal Chemistry[ISSN 0022-2623],v. 47 (11), p. 2877-2886
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