Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50474
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dc.contributor.authorDíaz-Chico, B. N.en_US
dc.contributor.authorOgasawara, Y.en_US
dc.contributor.authorChamness, G. C.en_US
dc.contributor.authorSalman, M.en_US
dc.contributor.authorMcGuire, W. L.en_US
dc.date.accessioned2018-11-24T16:19:22Z-
dc.date.available2018-11-24T16:19:22Z-
dc.date.issued1988en_US
dc.identifier.issn0022-4731en_US
dc.identifier.urihttp://hdl.handle.net/10553/50474-
dc.description.abstractA 65-kDa estrogen receptor (ER) protein has been demonst~ted both by sucrose gradient analysis and by immunoblot, using anti-ER monoclonal antibodies (MAbs). Since the ER is denatured in many experimental situations, such as formaldehyde fixing of samples for histochemistry and electroimmunoblotting studies, in this work we used a denatured 60-70-kDa ER-rich protein preparation as antigen for mice immunization in order to raise antiER MAbs. That material was obtained by affinity purification on an allyl-estradiol matrix of the MCF-7 cytosolic ER, followed by further isolation and enrichment by PAGE. NS-1 myeloma cells and spleen lymphocytes from the immunized mice were fused, and resultant hybridoma colonies were screened by [1251]-estradiol-labelled nuclear ER immunoprecipitation. The isolated MAb, E476, shows a moderate ability to precipitate ER and reacts strongly with a 46-kDa antigen in Western blot assay. The 46kDa antigen was not detectable in native cytosol but became reactive after 50% ammonium sulfate precipitation of cytosolic proteins. The 46-kDa antigen appeared concentrated in the NaSCN plus estradiol eluate of the affinity column used for cytosolic ER purification. Freshly prepared 60-70-kDa material from the preparative gel electrophoresis did not show any E476 reactivity. However, when the 60-70-kDa proteins were frozen, thawed and speed vacuum concentrated, the 46-kDa antigen became detectable. Storage increased the reactivity of the 60-70-kDa material with the E476 MAb. The 46 kDa antigen was present only in the ER positive cell lines, and was absent in all negative cell lines tested. The 46-kDa protein is also present in the ER positive human breast cancer specimens. We conclude that the 46-kDa protein identified with the E476 MAb in human breast cancer is probably a naturally occurring ER fragment.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Steroid Biochemistryen_US
dc.sourceJournal of Steroid Biochemistry[ISSN 0022-4731],v. 30, p. 315-320en_US
dc.subject32 Ciencias médicasen_US
dc.subject320101 Oncologíaen_US
dc.subject2302 Bioquímicaen_US
dc.subject.other46-kDa antigenen_US
dc.subject.otherEstrogen receptoren_US
dc.subject.otherBreast canceren_US
dc.titleA 46-kDa antigen associated with estrogen receptor in human breast canceren_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/0022-4731(88)90114-8en_US
dc.identifier.scopus0023690253-
dc.contributor.authorscopusid7003603506-
dc.contributor.authorscopusid57197976756-
dc.contributor.authorscopusid7003540569-
dc.contributor.authorscopusid7102542261-
dc.contributor.authorscopusid7201836333-
dc.description.lastpage320en_US
dc.description.firstpage315en_US
dc.relation.volume30en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages6en_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameDíaz Chico, Bonifacio-
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