Identificador persistente para citar o vincular este elemento:
http://hdl.handle.net/10553/50474
Campo DC | Valor | idioma |
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dc.contributor.author | Díaz-Chico, B. N. | en_US |
dc.contributor.author | Ogasawara, Y. | en_US |
dc.contributor.author | Chamness, G. C. | en_US |
dc.contributor.author | Salman, M. | en_US |
dc.contributor.author | McGuire, W. L. | en_US |
dc.date.accessioned | 2018-11-24T16:19:22Z | - |
dc.date.available | 2018-11-24T16:19:22Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.issn | 0022-4731 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/50474 | - |
dc.description.abstract | A 65-kDa estrogen receptor (ER) protein has been demonst~ted both by sucrose gradient analysis and by immunoblot, using anti-ER monoclonal antibodies (MAbs). Since the ER is denatured in many experimental situations, such as formaldehyde fixing of samples for histochemistry and electroimmunoblotting studies, in this work we used a denatured 60-70-kDa ER-rich protein preparation as antigen for mice immunization in order to raise antiER MAbs. That material was obtained by affinity purification on an allyl-estradiol matrix of the MCF-7 cytosolic ER, followed by further isolation and enrichment by PAGE. NS-1 myeloma cells and spleen lymphocytes from the immunized mice were fused, and resultant hybridoma colonies were screened by [1251]-estradiol-labelled nuclear ER immunoprecipitation. The isolated MAb, E476, shows a moderate ability to precipitate ER and reacts strongly with a 46-kDa antigen in Western blot assay. The 46kDa antigen was not detectable in native cytosol but became reactive after 50% ammonium sulfate precipitation of cytosolic proteins. The 46-kDa antigen appeared concentrated in the NaSCN plus estradiol eluate of the affinity column used for cytosolic ER purification. Freshly prepared 60-70-kDa material from the preparative gel electrophoresis did not show any E476 reactivity. However, when the 60-70-kDa proteins were frozen, thawed and speed vacuum concentrated, the 46-kDa antigen became detectable. Storage increased the reactivity of the 60-70-kDa material with the E476 MAb. The 46 kDa antigen was present only in the ER positive cell lines, and was absent in all negative cell lines tested. The 46-kDa protein is also present in the ER positive human breast cancer specimens. We conclude that the 46-kDa protein identified with the E476 MAb in human breast cancer is probably a naturally occurring ER fragment. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Steroid Biochemistry | en_US |
dc.source | Journal of Steroid Biochemistry[ISSN 0022-4731],v. 30, p. 315-320 | en_US |
dc.subject | 32 Ciencias médicas | en_US |
dc.subject | 320101 Oncología | en_US |
dc.subject | 2302 Bioquímica | en_US |
dc.subject.other | 46-kDa antigen | en_US |
dc.subject.other | Estrogen receptor | en_US |
dc.subject.other | Breast cancer | en_US |
dc.title | A 46-kDa antigen associated with estrogen receptor in human breast cancer | en_US |
dc.type | info:eu-repo/semantics/article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/0022-4731(88)90114-8 | en_US |
dc.identifier.scopus | 0023690253 | - |
dc.contributor.authorscopusid | 7003603506 | - |
dc.contributor.authorscopusid | 57197976756 | - |
dc.contributor.authorscopusid | 7003540569 | - |
dc.contributor.authorscopusid | 7102542261 | - |
dc.contributor.authorscopusid | 7201836333 | - |
dc.description.lastpage | 320 | en_US |
dc.description.firstpage | 315 | en_US |
dc.relation.volume | 30 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.description.numberofpages | 6 | en_US |
dc.utils.revision | Sí | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-MED | en_US |
item.fulltext | Sin texto completo | - |
item.grantfulltext | none | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.orcid | 0000-0001-5633-6185 | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | Díaz Chico, Bonifacio | - |
Colección: | Artículos |
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