Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50468
Title: Immunohistochemical detection of Dirofilaria immitis using a rabbit polyclonal anti-serum against adult somatic antigens and excretory-secretory products
Authors: Molina Caballero, José Manuel 
Orós Montón, Jorge Ignacio 
Ferrer Quintana, Otilia Rosa 
Rodrigues, E.
Gutierrez, A. C.
Hernández, S.
UNESCO Clasification: 32 Ciencias médicas
320712 Parasitología
Issue Date: 1997
Journal: Parassitologia 
Abstract: An immunohistochemical study on tissue sections of kidney, lung, liver and spleen from six dogs naturally infected by Dirofilaria immitis was carried out, using a rabbit polyclonal anti-serum against somatic antigen (SA) of adult worms, excretory-secretory products (ES) of adult worms and fraction C3 of complement (Dog C3). Positive immunoreactions were observed on circulating microfilariae when anti-SA and anti-ES were used, but no differences were noted between the two anti-sera. No antigen deposits were observed when the three anti-sera (anti-SA, anti-ES or anti-C3) were employed as primary antibody, in two cases of human filariosis (by Loa loa and Mansonella streptocerca) no positive immunoreactions were observed.
URI: http://hdl.handle.net/10553/50468
ISSN: 0048-2951
Source: Parassitologia[ISSN 0048-2951],v. 39, p. 441-444
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