1. accedaCRIS
  2. Publicaciones
  3. Artículos
Please use this identifier to cite or link to this item: https://accedacris.ulpgc.es/jspui/handle/10553/50002
DC FieldValueLanguage
dc.contributor.authorHattori, Masahiraen_US
dc.contributor.authorTugores, Antonioen_US
dc.contributor.authorVeloz, Lindaen_US
dc.contributor.authorBrenner, David A.en_US
dc.contributor.authorKarin, Michaelen_US
dc.date.accessioned2018-11-24T12:28:09Z-
dc.date.available2018-11-24T12:28:09Z-
dc.date.issued1990en_US
dc.identifier.issn1044-5498en_US
dc.identifier.urihttps://accedacris.ulpgc.es/handle/10553/50002-
dc.description.abstractWe have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.en_US
dc.languageengen_US
dc.relation.ispartofDNA and Cell Biologyen_US
dc.sourceDNA and Cell Biology[ISSN 1044-5498],v. 9(10), p. 777-781 (Diciembre 1990)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320102 Genética clínicaen_US
dc.subject2407 Biología celularen_US
dc.subject.otherNuclear extractsen_US
dc.subject.otherPlasmid DNAen_US
dc.subject.otherDNA binding proteinsen_US
dc.titleA Simplified Method for the Preparation of Transcriptionally Active Liver Nuclear Extractsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1089/dna.1990.9.777en_US
dc.identifier.scopus0025647389-
dc.contributor.authorscopusid7401881968-
dc.contributor.authorscopusid6701671839-
dc.contributor.authorscopusid6508090498-
dc.contributor.authorscopusid13605766800-
dc.contributor.authorscopusid36040291000-
dc.description.lastpage781en_US
dc.description.firstpage777en_US
dc.relation.volume9en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages5en_US
dc.utils.revisionSíen_US
dc.date.coverdateDiciembreen_US
dc.identifier.ulpgcSíen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0002-1849-9239-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameTugores Céster, Antonio-
Appears in Collections:Artículos
Show simple item record

SCOPUSTM   
Citations

125
checked on Jun 8, 2025

WEB OF SCIENCETM
Citations

135
checked on Jan 25, 2026

Page view(s)

188
checked on Jan 9, 2026

Google ScholarTM

Check

Altmetric


Share


Export metadata


Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.