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Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50002
DC FieldValueLanguage
dc.contributor.authorHattori, Masahiraen_US
dc.contributor.authorTugores, Antonioen_US
dc.contributor.authorVeloz, Lindaen_US
dc.contributor.authorBrenner, David A.en_US
dc.contributor.authorKarin, Michaelen_US
dc.date.accessioned2018-11-24T12:28:09Z-
dc.date.available2018-11-24T12:28:09Z-
dc.date.issued1990en_US
dc.identifier.issn1044-5498en_US
dc.identifier.urihttp://hdl.handle.net/10553/50002-
dc.description.abstractWe have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.en_US
dc.languageengen_US
dc.relation.ispartofDNA and Cell Biologyen_US
dc.sourceDNA and Cell Biology[ISSN 1044-5498],v. 9(10), p. 777-781 (Diciembre 1990)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320102 Genética clínicaen_US
dc.subject2407 Biología celularen_US
dc.subject.otherNuclear extractsen_US
dc.subject.otherPlasmid DNAen_US
dc.subject.otherDNA binding proteinsen_US
dc.titleA Simplified Method for the Preparation of Transcriptionally Active Liver Nuclear Extractsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1089/dna.1990.9.777en_US
dc.identifier.scopus0025647389-
dc.contributor.authorscopusid7401881968-
dc.contributor.authorscopusid6701671839-
dc.contributor.authorscopusid6508090498-
dc.contributor.authorscopusid13605766800-
dc.contributor.authorscopusid36040291000-
dc.description.lastpage781en_US
dc.description.firstpage777en_US
dc.relation.volume9en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages5en_US
dc.utils.revisionSíen_US
dc.date.coverdateDiciembreen_US
dc.identifier.ulpgcSíen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0002-1849-9239-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameTugores Céster,Antonio-
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