Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/49969
DC FieldValueLanguage
dc.contributor.authorPerilli, Mariagraziaen_US
dc.contributor.authorEttorre, Danielaen_US
dc.contributor.authorSegatore, Bernardettaen_US
dc.contributor.authorCaporale, Bibianaen_US
dc.contributor.authorSantis, Francesca Deen_US
dc.contributor.authorPochetti, Giorgioen_US
dc.contributor.authorBrigante, Giocondaen_US
dc.contributor.authorMazza, Fernandoen_US
dc.contributor.authorTavìo, Marìa M.en_US
dc.contributor.authorAmicosante, Gianfrancoen_US
dc.date.accessioned2018-11-24T12:12:21Z-
dc.date.available2018-11-24T12:12:21Z-
dc.date.issued2004en_US
dc.identifier.issn0378-1097en_US
dc.identifier.urihttp://hdl.handle.net/10553/49969-
dc.description.abstractAn efficient over-expression system was developed for CTX-M-3 extended-spectrum-beta-lactamase. The recombinant enzyme was purified from 11 of culture to yield 22 mg of pure enzyme. The N-terminal amino acid sequence was determined to be NH2-QTADVQ... Determination of kinetic parameters with the purified CTX-M-3 revealed efficient hydrolysis of penicillins and cephalosporins, while ceftazidime and aztreonam were very poor substrates. Clavulanic acid, sulbactam and especially tazobactam inhibited the CTX-M-3 enzyme.en_US
dc.languageengen_US
dc.relation.ispartofFEMS Microbiology Lettersen_US
dc.sourceFEMS Microbiology Letters[ISSN 0378-1097],v. 241, p. 229-232en_US
dc.subject32 Ciencias médicasen_US
dc.subject320103 Microbiología clínicaen_US
dc.subject.otherKlebsiella-Pneumoniaeen_US
dc.subject.otherEnterobacteriaceaeen_US
dc.subject.otherEnzymeen_US
dc.subject.otherIdentificationen_US
dc.subject.otherProteinsen_US
dc.subject.otherPolanden_US
dc.subject.otherSpainen_US
dc.titleOverexpression system and biochemical profile of CTX-M-3 extended-spectrum β-lactamase expressed in Escherichia colien_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.femsle.2004.10.031en_US
dc.identifier.scopus10444250255-
dc.identifier.isi000226174000015-
dc.contributor.authorscopusid7004624865-
dc.contributor.authorscopusid6506612774-
dc.contributor.authorscopusid6603452179-
dc.contributor.authorscopusid6506603608-
dc.contributor.authorscopusid7801420086-
dc.contributor.authorscopusid6603710609-
dc.contributor.authorscopusid6507564767-
dc.contributor.authorscopusid56214236100-
dc.contributor.authorscopusid6701659492-
dc.contributor.authorscopusid7006729208-
dc.description.lastpage232en_US
dc.description.firstpage229en_US
dc.relation.volume241en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid358961-
dc.contributor.daisngid7075886-
dc.contributor.daisngid960054-
dc.contributor.daisngid9776903-
dc.contributor.daisngid712896-
dc.contributor.daisngid558742-
dc.contributor.daisngid2264475-
dc.contributor.daisngid1663101-
dc.contributor.daisngid7853284-
dc.contributor.daisngid144499-
dc.description.numberofpages4en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Perilli, M-
dc.contributor.wosstandardWOS:Ettorre, D-
dc.contributor.wosstandardWOS:Segatore, B-
dc.contributor.wosstandardWOS:Caporale, B-
dc.contributor.wosstandardWOS:De Santis, F-
dc.contributor.wosstandardWOS:Pochetti, G-
dc.contributor.wosstandardWOS:Brigante, G-
dc.contributor.wosstandardWOS:Mazza, F-
dc.contributor.wosstandardWOS:Tavio, MM-
dc.contributor.wosstandardWOS:Amicosante, G-
dc.date.coverdateDiciembre 2004en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr1,84-
dc.description.jcrqQ3-
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Investigación Básica y Aplicada en Ciencias de la Salud-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0002-1808-7461-
crisitem.author.parentorgDepartamento de Ciencias Clínicas-
crisitem.author.fullNameTavío Pérez, María Del Mar-
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