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Title: Antimicrobial susceptibility of Enterococcus strains isolated from poultry faeces
Authors: Tejedor-Junco, M. T. 
Afonso-Rodríguez, O.
Martín Barrasa, José Luis 
González Martín, Margarita Rosa 
UNESCO Clasification: 310907 Patología
241404 Bacteriología
Keywords: Enterococcus
Antimicrobial resistance
Issue Date: 2005
Journal: Research in Veterinary Science 
Abstract: We have investigated the resistance of Enterococcus isolated from poultry faeces to antibiotics commonly used as therapy of enterococcal infections. Identification was made by the method of Facklam and Collins. Minimal inhibitory concentrations of penicillin, ampicillin, vancomycin and teicoplanin were determined and high level aminoglycoside resistance was investigated. Genes codifying high level aminoglycoside resistance (HLAR) were determined by PCR. Fifty five Enterococcus strains were isolated (63.6% E. faecalis, 12.7% E. mundtii, 9.1% E. faecium, 7.3% E. casseliflavus, 3.7% E. durans and 3.6% E. hirae ). None of the strains were resistant to VAN, TEC, P or AM. HLAR was found in 34.5% of strains for SM, 27.3% for KM and 7.3% for GM. The gene for the bifunctional enzyme was found only in one strain, that showed HLAR to GM and KM. Fourteen strains harboured the gene aph (3′)-III, being 11 resistant to KM and STR, and three resistant to GM, KM and STR. The remaining six strains showed HLAR to STR, but were negative for the three genes tested by PCR. The gene ant (4′4″) was not detected in any of the strains. No unexpected vancomycin resistance was detected. The resistance rates among poultry strains were lower than those found among human strains isolated from hospital patients in recent Canary studies.
ISSN: 0034-5288
DOI: 10.1016/j.rvsc.2004.05.008
Source: Research in Veterinary Science [ISSN 0034-5288], v. 78 (1), p. 33-38
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