Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/49341
Title: Aminoglycoside resistance mechanisms in clinical isolates of Pseudomonas aeruginosa from the Canary Islands
Authors: Esparragon, FR 
González Martín, Margarita 
González Lama, Zoilo
Sabatelli, Frank J.
Tejedor Junco, María Teresa 
UNESCO Clasification: 32 Ciencias médicas
320103 Microbiología clínica
Keywords: Outer-Membrane
Gentamicin
Gene
Profiles
Issue Date: 2000
Journal: Zentralblatt für Bakteriologie : international journal of medical microbiology 
Abstract: Strains of Pseudomonas aeruginosa resistant to aminoglycoside antibiotics were selected from 152 clinical isolates. We identified two patterns of resistance correlating with the resistance mechanism characterized by changes in permeability, enzymatic modification due to the acetylating enzyme, AAC(6')-II, or a combination of both. We detected enzymatic activity of the phosphorylase enzyme, APH(3'), in all the isolates. We compared the mechanisms of resistance detected by three methods i.e., radioenzymatic assay, phenotype of resistance and DNA probes. The phenotype of resistance was tested using a kit developed by Schering-Plough Corp. Hybridization was made with 18 DNA probes for the most frequent genes encoding for aminoglycoside-modifying enzymes. All isolates with AAC(6') activity hybridized with the aac(6')-Ib probes and to a minor degree, with the aac(6')-IIb probe. None of the isolates showed hybridization with aph(3')-I, aph(3')-II, or aph(3')-III DNA probes. Serotyping of the strains showed that the O:11 serotype was the most frequent one in strains whose resistance was due to the AAC(6') enzyme. The O:6 serotype was associated with changes in permeability. Encoding of the resistance mechanism seemed to be chromosomal in all the strains.
URI: http://hdl.handle.net/10553/49341
ISSN: 0934-8840
Source: Zentralblatt Fur Bakteriologie-International Journal Of Medical Microbiology Virology Parasitology And Infectious Diseases[ISSN 0934-8840],v. 289 (8), p. 817-826
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