|Title:||Homocysteinylated protein levels in internal mammary artery (IMA) fragments and its genotype-dependence. S-Homocysteine-induced methylation modifications in IMA and aortic fragments||Authors:||Rodriguez-Esparragon, Francisco
Serna-Gómez, Jaime Alberto
García-Bello, Miguel A.
Rodriguez-Perez, Jose C.
|UNESCO Clasification:||32 Ciencias médicas
320102 Genética clínica
Endothelial-Cells, et al
|Issue Date:||2012||Journal:||Molecular and Cellular Biochemistry||Abstract:||The resistance of internal mammary artery (IMA) toward atherosclerosis is not well understood. In plasma, homocysteine (Hcy) occurs in reduced, oxidized, homocysteine thiolactone and a component of proteins as a result of N- or S-homocysteinylation. We evaluated S/N-homocysteinylated protein levels in IMA fragments of patients undergoing coronary artery bypass grafting, and whether they were affected by genetic common variants. We tested whether tHcy, Hcy-S-protein levels, genotypes or Hcy-induced methylation modifications were related to differences in iNOS, Ddah2, and eNOS gene expression between territories. A small percentage of Hcy-S-proteins were found in IMA fragments. The Mthfr C677T (rs1801133) and Pon-1 Leu55Met (rs854560) variants were associated with Hcy-S-proteins. We observed a gradual difference according to Hcy-S-protein levels in the methylation degree of the Ddah2 gene promoter in aortic, but not in IMA, fragments. No correlation between the degree of methylation and the Ddah2 gene expression levels was found in both types of analyzed fragments. Total Hcy but not Hcy-S-proteins correlated with iNOS promoter methylation. Analyzed variants seem to contribute to the in vivo Hcy binding properties to IMA. The contribution of the Hcy-derived methylation modifications to Ddah2 and eNOS gene expression seems to be tissue-specific and independent of the Ddah2/ADMA/eNOS pathway. Hcy-derived methylation modifications to the iNOS gene promoter contribute to a lesser extent to iNOS gene expression.||URI:||http://hdl.handle.net/10553/49318||ISSN:||0300-8177||DOI:||10.1007/s11010-012-1387-7||Source:||Molecular And Cellular Biochemistry[ISSN 0300-8177],v. 369 (1-2), p. 235-246|
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