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Title: Early pregnancy diagnosis in goats by determination of pregnancy- associated glycoprotein concentrations in plasma samples
Authors: González, F.
Sulon, J.
Garbayo, J. M.
Batista, M. 
Cabrera, F. 
Calero, P. 
Gracia, A. 
Beckers, J. F.
Keywords: Progesterone Concentrations
Ultrasonography, et al
Issue Date: 1999
Publisher: 0093-691X
Journal: Theriogenology 
Abstract: Different RIA systems available for measuring the concentrations of pregnancy-associated glycoproteins (PAGs) in dairy goats were compared in order to evaluate their accuracy in early pregnancy diagnosis. Plasma concentrations of PAGs were determined by 3 heterologous RIA systems with a bovine PAG standard and tracer in combination with antisera anti-ovine PAG (RIA 1), anti-caprine PAG(55+62) (RIA 2), anti-caprine PAG(55+59) (RIA 3), and by 2 homologous RIA systems that employed caprine PAG(55+62) and caprine PAG(55+59) and their specific antisera (RIAs 4 and 5, respectively). In all of the RIAs, the mean concentrations of PAGs were significantly higher (P<0.01) in pregnant than in nonpregnant goats from Day 21 onwards after breeding. On Day 21, the accuracy rates of early pregnancy diagnoses were 56% (RIA 1), 96% (RIA 2), 99% (RIA 3), 95% (RIA 4) and 90% (RIA 5), whereas on Day 28 these rates were >99% for RIAs 2, 3, 4 and 5. The RIAs for PAGs depend on proteins from the placenta being present in maternal plasma and require only a single sample of blood, to distinguish pregnant goats from those that fail to return to estrus for other reasons. The homologous and semi-heterologous assays are highly accurate as early as Day 21 of pregnancy. (C) 1999 by Elsevier Science Inc.
ISSN: 0093-691X
DOI: 10.1016/S0093-691X(99)00165-X
Source: Theriogenology[ISSN 0093-691X],v. 52, p. 717-725
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