Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/48641
Campo DC Valoridioma
dc.contributor.authorMartín-Fernández, José M.en_US
dc.contributor.authorCabanillas, Juan A.en_US
dc.contributor.authorRivero-Carmena, Miguelen_US
dc.contributor.authorLacasa, Estheren_US
dc.contributor.authorPardo, Juliánen_US
dc.contributor.authorAnel, Albertoen_US
dc.contributor.authorRamírez-Duque, Pedro R.en_US
dc.contributor.authorMerino, Fernandoen_US
dc.contributor.authorRodríguez-Gallego, Carlosen_US
dc.contributor.authorRegueiro, José R.en_US
dc.date.accessioned2018-11-23T23:39:43Z-
dc.date.available2018-11-23T23:39:43Z-
dc.date.issued2005en_US
dc.identifier.issn0741-5400en_US
dc.identifier.urihttp://hdl.handle.net/10553/48641-
dc.description.abstractCytolytic CD8+ T lymphocytes are the main cell type involved in the fatal lymphoproliferative-accelerated phase of the Chediak-Higashi syndrome (CHS). To generate a cellular tool to study the defects of this T cell subset in vitro, we have used Herpesvirus saimiri, a lymphotropic virus that transforms human T lymphocytes into extended growth and in addition, endows them with natural killer (NK) features. Transformed CHS CD8+ T cells were generated and characterized in comparison with healthy controls. The results showed that transformed CHS T cells maintained the defects described in primary CHS lymphocytes, such as giant secretory lysosomes and impaired NK and T cell receptor/CD3-induced, perforin-mediated cytolytic activity [which, however, could be restored after extended culture in the presence of interleukin-2 (IL-2)]. Upon activation with phorbol ester plus calcium ionophore or upon extended culture with IL-2, transformed CHS T cells showed normal, perforin-independent plasma membrane CD178/CD95L/FasL-mediated cytolytic activity but negligible secretion of microvesicle-bound CD95L. Transformed (and primary) CHS T cells were otherwise normal for cytolysis-independent activation functions, such as proliferation, surface expression of several activation markers including major histocompatibility complex class II, and cytokine or surface activation-marker induction. Therefore, the CHS protein [CHS1/LYST (for lysosomal traffic regulator)] can be dispensable for certain NK and T cell cytolytic activities of activated CHS CD8+ T lymphocytes, but it seems to be required for microvesicle secretion of CD95L. We conclude that transformed CHS T cells may be useful as a tool to study in vitro the relative role of CHS1/LYST in NK and T lymphocyte cytolysis and antigen presentation.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Leukocyte Biologyen_US
dc.sourceJournal of Leukocyte Biology[ISSN 0741-5400],v. 77, p. 661-668 (Mayo 2005)en_US
dc.subject32 Ciencias médicasen_US
dc.subject3205 Medicina internaen_US
dc.subject.otherT lymphocyteen_US
dc.subject.otherImmunodeficiency diseasesen_US
dc.subject.otherCytolytic cellen_US
dc.titleHerpesvirus saimiri-transformed CD8<sup>+</sup>T cells as a tool to study Chediak-Higashi syndrome cytolytic lymphocytesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1189/jlb.0904500en_US
dc.identifier.scopus20944443185-
dc.contributor.authorscopusid7004615778-
dc.contributor.authorscopusid6603228931-
dc.contributor.authorscopusid8516237900-
dc.contributor.authorscopusid11639005500-
dc.contributor.authorscopusid57197457384-
dc.contributor.authorscopusid7003516028-
dc.contributor.authorscopusid6507970210-
dc.contributor.authorscopusid57201314592-
dc.contributor.authorscopusid6602114379-
dc.contributor.authorscopusid7005510950-
dc.description.lastpage668en_US
dc.description.firstpage661en_US
dc.relation.volume77en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.date.coverdateMayo 2005en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr4,627-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.orcid0000-0002-4344-8644-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameRodríguez Gallego, José Carlos-
Colección:Artículos
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