Induction of G(2)-M phase arrest and apoptosis by alpha-methylene-gamma-butyrolactones in human leukemia cells

Título:	Induction of G(2)-M phase arrest and apoptosis by alpha-methylene-gamma-butyrolactones in human leukemia cells
Autores/as:	Torres, Fernando Quintana, José Cabrera, Javier Loro, Juan F. León, Francisco Bermejo, Jaime Estévez, Francisco
Clasificación UNESCO:	3205 Medicina interna
Palabras clave:	α-Methylene-γ-butyrolactones Apoptosis Caspases DNA fragmentation PARP cleavage, et al.
Fecha de publicación:	2008
Editor/a:	0304-3835
Proyectos:	Desarrollo de Nuevos, Mas Seguros y Mas Efectivos Compuestos Antileucemicos Nuevos Compuestos Antileucémicos
Publicación seriada:	Cancer Letters
Resumen:	In this study, we investigated the effect of three synthetic α-methylene-γ-butyrolactones (MBL) on viability of 10 human tumor cell lines and found that these lactones were highly cytotoxic against leukemia cells. Studies performed on HL-60 cells indicate that MBL induce G2-M arrest and apoptosis through a caspase-dependent mechanism. Apoptosis was associated to cytochrome c release, cleavage of procaspases-9 and -3, and hydrolysis of PARP. Intracellular reactive oxygen species (ROS) seem to play a key role since high levels of ROS were produced early (<15 min) and apoptosis was completely abrogated by the free radical scavenger N-acetyl-l-cysteine (NAC).
URI:	http://hdl.handle.net/10553/48405
ISSN:	0304-3835
DOI:	10.1016/j.canlet.2008.04.028
Fuente:	Cancer Letters [ISSN 0304-3835], v. 269 (1), p. 139-147
Colección:	Artículos

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