Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/48287
Title: Factors affecting protoplast yield of the carrageenophyte Solieria filiformis (Gigartinales, Rhodophyta)
Authors: Pinchetti, Juan Luis Gómez 
Björk, Mats
Pedersén, Marianne
Reina, Guillermo García 
UNESCO Clasification: 251004 Botánica marina
241707 Algología (ficología)
Keywords: Protoplast
Solieria filiformis
Enzymes
Haliotis coccinea canariensis
Culture
Issue Date: 1993
Publisher: 0721-7714
Journal: Plant Cell Reports 
Abstract: The effect of age, pH of the culture medium, pre-treatment of tissues, enzymes sources and enzymatic adaptability of phycophages fed with a monospecific diet were analyzed on the protoplast yields of the red seaweed Solieria filiformis (Kützing) Gabrielson. New apices from fast growing plants showed the highest protoplast yields. The protoplast yield decreased when the pH of the culture medium increased from 6.0 to 9.0. Crude extracts from the abalone Haliotis coccinea canariensis Nordsieck, fed with Solieria filiformis thalli for three months in combination with cellulysin, released the highest number of viable cells and protoplasts. Yields ranged from 1.0 to 8.5 x 106 protoplasts per gram of fresh weight.
URI: http://hdl.handle.net/10553/48287
ISSN: 0721-7714
DOI: 10.1007/BF00233056
Source: Plant Cell Reports [ISSN 0721-7714], v. 12, p. 541-545
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