Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/48287
Título: Factors affecting protoplast yield of the carrageenophyte Solieria filiformis (Gigartinales, Rhodophyta)
Autores/as: Pinchetti, Juan Luis Gómez 
Björk, Mats
Pedersén, Marianne
Reina, Guillermo García 
Clasificación UNESCO: 251004 Botánica marina
241707 Algología (ficología)
Palabras clave: Protoplast
Solieria filiformis
Enzymes
Haliotis coccinea canariensis
Culture
Fecha de publicación: 1993
Editor/a: 0721-7714
Publicación seriada: Plant Cell Reports 
Resumen: The effect of age, pH of the culture medium, pre-treatment of tissues, enzymes sources and enzymatic adaptability of phycophages fed with a monospecific diet were analyzed on the protoplast yields of the red seaweed Solieria filiformis (Kützing) Gabrielson. New apices from fast growing plants showed the highest protoplast yields. The protoplast yield decreased when the pH of the culture medium increased from 6.0 to 9.0. Crude extracts from the abalone Haliotis coccinea canariensis Nordsieck, fed with Solieria filiformis thalli for three months in combination with cellulysin, released the highest number of viable cells and protoplasts. Yields ranged from 1.0 to 8.5 x 106 protoplasts per gram of fresh weight.
URI: http://hdl.handle.net/10553/48287
ISSN: 0721-7714
DOI: 10.1007/BF00233056
Fuente: Plant Cell Reports [ISSN 0721-7714], v. 12, p. 541-545
Colección:Artículos
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