Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47717
Title: Stem cells protect the bronchial stump in rat, increasing Sox6, Col2a1, and Agc1 expression
Authors: Llontop, Pedro
Santana-Rodríguez, Norberto
Clavo, Bernardino 
Quintana, Ardiel
Fiuza, María D.
Camacho, Rafael
Santana-Rodríguez, Alfredo 
Santana, Carlos
Ruíz-Caballero, José A.
Keywords: Differentiation
Cartilage
Survival
Collagen
Fistula
Issue Date: 2014
Publisher: 0341-2040
Journal: Lung 
Abstract: Post-pneumonectomy bronchopleural fistulas (BPFs) are associated with high morbidity and mortality. Currently, since the management of BPFs is difficult to assess, the best therapeutic approach is prevention. Our objective was to evaluate the effects of adipose-derived stem cells (ASCs) on the healing of the bronchial stump in an experimental animal model.Left pneumonectomy was performed in 37 Sprague-Dawley rats. Animals were randomly assigned to a control group (n = 13), an ASC group (n = 12), and an ASC plus Tissucol(A (R)) group (ASCT) (n = 12). The ASCs and ASCTs were locally administered at the bronchial stump after surgical pneumonectomy. Animals were killed at 10 and 20 days. We analyzed histological changes and changes in the expression of relevant genes involved in wound repair in the bronchial stump.Two control animals, one animal from the ASC group, and one from the ASCT group died from early BPF. All the remaining animals had an adequate postoperative outcome. ASCs and ASCTs significantly decreased the necrosis and ulcerations of the bronchial stump at 10 and 20 days. ASCs significantly decreased mRNA expression of Igf1 at 10 days and Igf1, Tgfb1, Vegfa, and Col2a1 at 20 days, whereas there was increased expression of Agc1 and Col2a1 at 10 days and Sox6 at 20 days.Our findings indicate that local ASCs protected the bronchial stump after pneumonectomy and induced local changes in gene expression related to their protective action. These results could lead to a potential new therapeutic modality for the prevention of BPF.
URI: http://hdl.handle.net/10553/47717
ISSN: 0341-2040
DOI: 10.1007/s00408-014-9569-6
Source: Lung[ISSN 0341-2040],v. 192, p. 441-448
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

2
checked on Aug 1, 2021

WEB OF SCIENCETM
Citations

1
checked on Aug 1, 2021

Page view(s)

10
checked on Jul 31, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.